Basic Information
Teacher Name : Kehe Huang
Job Title: professor
Phone：86-25-84395507 (work); 86-13605188392 (mobile)
Email：khhuang@njau.edu.cn
Address： College of Veterinary Medicine, No.1 Weigang, Xuanwu District, Nanjing Agricultural University, Nanjing 210095, China
Research Areas and Descriptors：
I have been working in teaching of Veterinary Internal Medicine and diagnosis and treatment of Veterinary Clinic. My study focuses on nutrient-immunity
interactions, nutritional and metabolic disorders and clinical nutrition of animals.
Education
03/91-11/94: Ph.D., Major in Veterinary Pathology, Nanjing Agricultural University, Nanjing 210095, China. Dissertation: Studies on effect of selenium on chicken's resistance against Marek's disease and its molecular mechanism.
09/85-07/88: MSc, Major in Veterinary Internal Medicine and Animal Nutrition, Nanjing Agricultural University, Nanjing 210095, China. Thesis: Diagnosis of selenium and/or vitamin E deficiency and their effects on immune responses in animals.
02/78-01/82: DVM, Major in Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China. Thesis: Epidemiological and pathological investigation of congenital tremor in newborn pigs.
Work Experience
12/99-present: Professor, Department of Clinical Sciences, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China. Worked on molecular nutrition and nutrient-immunity interactions.
09/13-11/13: Visiting Scholar in the Department of Animal Sciences, Cornell University, Ithaca,
USA
. Worked on molecular nutrition of animals.
06/11-08/11: Visiting Scholar in the Department of Animal & Food Sciences, University of Kentucky, Lexington, USA. Worked on molecular nutrition of animals.
12/08-05/09: Visiting Scholar in Institute for Cell & Molecular Biosciences, The Medical School, Framlington Place, NEWCASTLE-UPON-TYNE, NE2 4HH,
UK
. Worked on molecular nutrition of animals.
10/01-04/02: Visiting Scholar in College of Veterinary Medicine, Tufts University, Grafton,
USA
. Worked on relationship between nutrition and parasites.
05/98-11/99: Associate Professor, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095,China. Worked on specific immunologic methods for diagnostic of Cryptosporidiosis and free radical biology of Cryptosordium parvum.
6/96-4/98: Postdoctoral fellow in Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan,
USA
. Worked on developing and validating small animal models and cell culture systems for the protozoan parasite Cryptosporidium parvum.
1/96-6/96: Visiting Scientist in United States Department of Agriculture, Agricultural Research Service, Avian Disease and Oncology Laboratory, East Lansing,
USA
. Worked on sequencing of Marek's disease virus genome by PCR amplification of its DNA.
12/94-1/96: Associate Professor, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China. Worked on molecular mechanism of increasing nonspecific resistance to infectious diseases of animals.
1/87-11/94: Assistant Professor, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095,China. Studied on disorders of nutrition and metabolism of animals and relations between nutrition, immunity and resistance against infection.
2/82-12/86: Researching assistant, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095,China. Focused on diagnosis, prevention and treatment of domestic animal and poultry diseases.
Awards and Group Memberships
Vice President of Veterinary Internal Medicine Branch of Chinese Association of Animal Husbandry and Veterinary Medicine since 2007.
First Technology Invention Prize awarded by the Commerce Ministry of China, 2015
Second Technology Invention Prize awarded by the Educational Ministry of China, 2012
Second Science and Technology Advancement Prize awarded by the government of Jiangsu Province, China, 2009
Second Science and Technology Advancement Prize awarded by the Educational Ministry of China, 2006
Government special allowance awarded by the State Council of China, 2002
Distinguished Expert awarded by the Agricultural Ministry of China, 1999
Second Science and Technology Advancement Prize awarded by the Agricultural Ministry of China, 1999
Third Science and Technology Advancement Prize awarded by the Educational Ministry of China, 1998
Fellowship of 6 months awarded by International Atomic Energy Agency, 1996
Research projects
2018 -2021: Grant awarded from National Natural Science Foundation of China: Intervention effect of Saccharomyces cerevisiae NAU - LYH - SGS on pig OTA poisoning and its mechanism. PI
2016 -2020: Grant awarded from the National Key R & D Program of China (2016YFD0501203): Prevention and control technology of trace elements, vitamins, and electrolyte imbalance in cattle and sheep. PI
2015 -2018: Grant awarded from National Natural Science Foundation of China: Role of selenoprotein S and its mode of action on selenium alleviation of ochratoxin A toxity in pigs. PI
2013 -2016: Grant awarded from National Natural Science Foundation of China: Seeking for key selenoproteins on regulation of immunity induced by selenium and regulation mechanism of the selenoprotein expressions in pigs. PI
2011-2012: Grant awarded from Ministry of Science and Technology of China: Preparation of a feeding additive for energy metabolic disease of dairy cows and its application. PI
2009 -2011: Grant awarded from National Natural Science Foundation of China: Application of a new type of organic selenium source on pigs and its molecular mechanism. PI
2008-2010: Grant awarded from Special Doctoral Program Funds of China: Application of a new type of organic selenium source on dairy cows and its molecular mechanism. PI
2007-2009: Grant awarded from National Natural Science Foundation of China: application of a new type of feeding additive on chickens and its mechanism PI
Publications
*Corresponding author
1． Kehe Huang, Wanfang Chen. Effect of selenium on resistance against Marek‘s disease and changes of the free radicals in chickens. Current Research in Marek’s disease. American Association of Avian Pathologists, 1996,486-491
2． Xiaolong Wang, Kehe Huang, et al. Chemical composition and microstructure of uroliths and urinary sediment crystals with feeding of cottonseed meal diet to water buftalo calves, Research in Veterinary Science 1997,62:275-280
3． Kehe Huang, Xiaolong Wang, et al. Pathogenesis of uroliths in Chinese swamp buffalo calves caused by feeding high level cottonseed meal diet, Buffalo Journal 1999,15(3): 292-301
4． Kehe Huang*, Shiguang Yang, Mark C. Healey. Effects of hydrogen peroxide on in vitro infections of Cryptosporidium parvum. Progress in Nature Science, 2001, 11(12): 946-951
5． Kehe Huang*, Shiguang Yang. Inhibitory effect of selenium on Cryptosporidium parvum infection in vitro and in vivo. Biological Trace Element Reseach, 2002, 90: 261-272
6． Kehe Huang, Donna E. Akiyoshi*, Xiaochuan Feng, and Saul Tzipori. Development of patent infection in immunosuppressed c57bl/6n mice with a single cryptosporidium meleagridis oocyst. Journal of Parasitology, 2003, 89(3)：620-622
7． Chenbo Yang, Yuan Jiang, Kehe Huang*, and Changqing, Zhu. Application of real-time PCR for detection of Campylobacter jejuni in poultry, milk and environmental water. FEMS Immunology and Medical Microbiology, 2003, 38（3）, 265-271
8． Yang C, Jiang Y, Huang K*, Zhu C, Yin Y, Gong JH, Yu H. A real-time PCR assay for the detection and quantitation of Campylobacter jejuni using SYBR Green I and the light cycler. The Yale Journal of Biology and Medicine, 2004, 77(5-6): 125-132.
9． X. Guo, K. Huang* And J. Tang. Clinicopathology of gout in growing layers induced by high calcium and high protein diets. British Poultry Science, 2005, 46(5): 641-646.
10．Tanxi Cai, Luyan Jiang, Chenbo Yang and Kehe Huang*. Application of real-time PCR for quantitative detection of Vibrio parahaemolyticus from seafood in eastern
China
. FEMS Immunology and Medical Microbiology, 2006，46（2）: 180 -185
11．Cuiling Pan, Kehe Huang*, Yuxin Zhao, Shunyi Qin, Fu Chen. Effect of Selenium Source and Level in Hen’s Diet on Tissue Selenium Deposition and Egg Selenium Concentrations. Journal of Agricultural and Food Chemistry, 2007, 55(3): 1027 - 1032
12．Shunyi Qin, Jianzhong Gao, Kehe Huang*. Effects of different selenium sources on the tissue selenium concentrations, blood GSH-PX activities and plasma interleukins cytokine levels in finishing lambs. Biological Trace Element Reseach，2007，116(1):91-102
13．Fu Chen, Kehe Huang*, Shunyi Qin, Yuxin Zhao, Cuiling Pan. Comparison of viability and infectivity of Cryptosporidium parvum oocysts stored in potassium dichromate solution and chlorinated tap water, Vet. Parasitol. 2007, 150: 13–17
14．Fu Chen, Kehe Huang*. Prevalence and phylogenetic analysis of Cryptosporidium in pigs in eastern
China
, Zoonoses and Public Health, 2007, 54: 393–400
15．Qunxing Pan, Kehe Huang*, Kongwang He, Fuzeng Lu. Effect of different selenium sources and levels on porcine circovirus type 2 replication in vitro. Journal of Trace Elements in Medicine and Biology, 2008，22：143–148
16．Qunxing Pan, Kongwang He, Kehe Huang. Development of recombinant porcine parvovirus-like particles as an antigen carrier formed by the hybrid vp2 protein carrying immunoreactive epitope of porcine circovirus type 2.Vaccine, 2008, 26：2119-2126
17．Xiaoquan Guo, Kehe Huang*, Fu Chen, Jianbing Luo, Cuiling Pan. High dietary calcium causes metabolic alkalosis in egg-type pullets, Poultry Sciences, 2008 , 87：1353-357
18．Jiajun Yang，Kehe Huang*，Shunyi Qin，Xianshi Wu，Zhiping Zhao，Fu Chen. Antibacterial Action of Selenium-Enriched Probiotics Against Pathogenic Escherichia coli. Dig Dis Sci 2009, 54：246-254
19．Shunyi Qin, Kehe Huang*, Jianzhong Gao, Da Huang, Tanxi Cai, Cuiling Pan. Comparison of glutathione peroxidase 1 and iodothyronine deiodinase 1 Mrna expression in murine liver after feeding selenite or selenized yeast. Journal of Trace Elements in Medicine and Biology, 2009，23：29–35
20． Zhong. Z, Wang Y, Qiao F, Wang Z, Du X, Xu J, Zhao J, Qu Q, Dong S, Sun Y, Huang L, Huang K*, Chen Z. Cytotoxicity of Brucella smooth strains for macrophages is mediated by increased secretion of the type IV secretion system. Microbiology, 2009, 155, 3392–3402
21．Xianshi Wu, Kehe Huang*, Chengwu Wei, Fu Chen, Cuiling Pan. Regulation of cellular glutathione peroxidase by different forms and concentrations of selenium in primary cultured bovine hepatocytes. Journal of Nutritional Biochemistry, 2010, 21:153–161
22．Xianshi Wu, Chengwu Wei, Cuiling Pan，Ying Duan and Kehe Huang*. Regulation of expression and activity of selenoenzymes by different forms and concentrations of selenium in primary cultured chicken hepatocytes. British Journal of Nutrition, 2010, 104:1605–1612
23．Chen W, Hu S, Qu L, Hu Q, Zhang Q, Zhi H, Huang K, Bu Z. A goat poxvirus-vectored peste-des-petits-ruminants vaccine induces long-lasting neutralization antibody to high levels in goats and sheep. Vaccine. 2010，28(30):4742-50
24．Linwu Ran, XianshiWu, Xiangzhen Shen, Kechun Zhang, Fei Ren, Kehe Huang*. Effects of selenium form on blood and milk selenium concentrations, milk component and milk fatty acid composition in dairy cows. J Sci Food Agric, 2010, 90(13):2214-2219.
25．Zhihua Ren, Zhiping Zhao, Yangguang Wang, Kehe Huang*. Preparation of selenium/zinc-enriched probiotics and their effect on blood selenium and zinc concentrations, antioxidant capacities, and intestina microflora in canine. Biol Trace Elem Res, 2011, 141(1-3):170-183.
26．Chen W, Cao W, Zhao H, Hu Q, Qu L, Hu S, Ge J, Wen Z, Wang X, Li H, Huang K*, Bu Z. Establishment of a stable CHO cell line with high level expression of recombinant porcine IFN-β. Cytokine. 2011, 54(3):324-9.
27．Ren Z, Pan C, Jiang L, Wu C, Liu Y, Zhong Z, Ran L, Ren F, Chen X, Wang Y, Zhu Y, Huang K*. Oxalate-degrading capacities of lactic acid bacteria in canine feces. Vet Microbiol. 2011，152(3-4):368-373.
28．Cuiling Pan, Yuxin Zhao, Shengfa F. Liao, Fu Chen, Shunyi Qin, Xianshi Wu, Hong Zhou, Kehe Huang*. Effect of selenium-enriched probiotics on laying performance, egg quality, egg selenium content, and egg glutathione peroxidase activity. Journal of Agricultural Food and Chemistry. 2011，59(21):11424-31
29．Chen X, Ren F, Hesketh J, Shi X, Li J, Gan F, Huang K*. Selenium blocks PCV2 replication promotion induced by oxidative stress via improving GPx1 expression. Free Radical Biology & Medicine. 2012, 53(3):395-405
30．Ren F, Chen X, Hesketh J, Gan F, Huang K*. Selenium promotes T-cell response to TCR-stimulation and ConA, but not PHA in primary porcine splenocytes. PLoS One. 2012, 7(4):e35375
31．Hu Q, Chen W, Huang K, Baron MD, Bu Z. Rescue of recombinant peste des petits ruminants virus: creation of a GFP-expressing virus and application in rapid virus neutralization test. Vet Res. 2012, 43(1):48
32．Chen X, Ren F, Hesketh J, Shi X, Li J, Gan F, Huang K*. Reactive oxygen species regulate the replication of porcine circovirus type 2 via NF-κB pathway. Virology, 2012, 426: 66-72.
33．Fu S, Xu J, Li X, Xie Y, Qiu Y, Du X, Yu S, Bai Y, Chen Y, Wang T, Wang Z, Yu Y, Peng G, Huang K, Huang L, Wang Y, Chen Z. Immunization of Mice with Recombinant Protein CobB or AsnC Confers Protection against Brucella abortus Infection. PLoS One. 2012, 7(2): e29552.
34．Li X, Xu J, Xie Y, Qiu Y, Fu S, Yuan X, Ke Y, Yu S, Du X, Cui M, Chen Y, Wang T, Wang Z, Yu Y, Huang K, Huang L, Peng G, Chen Z, Wang Y. Vaccination with recombinant flagellar proteins FlgJ and FliN induce protection against Brucella abortus 544 infection in BALB/c mice. Vet Microbiol. 2012, 161(1-2):137-44.
35．Chen F, Huang K*. Effects of the Chinese medicine matrine on experimental C. parvum infection in BALB/c mice and MDBK cells. Parasitol Res. 2012, 111(4):1827-32.
36．Chen F, Huang K*. Prevalence and molecular characterization of Cryptosporidium spp. in dairy cattle from farms in
China
. Journal of Veterinary Science, 2012, 13(1):15-22.
37．Ibrahim HA, Zhu Y, Wu C, Lu C, Ezekwe MO, Liao SF, Huang K*. Selenium-Enriched Probiotics Improves Murine Male Fertility Compromised by High Fat Diet. Biol Trace Elem Res, 2012, 147(1-3):428.
38．Huang J, Cao L, Guo W, Yuan R, Jia Z, Huang K*. Enhanced soluble expression of recombinant Flavobacterium heparinum heparinase I in Escherichia coli by fusing it with various soluble partners. Protein Expr Purif. 2012, 83(2):169-76.
39．Gan F, Ren F, Chen X, Lv C, Pan C, Ye G, Shi J, Shi X, Zhou H, Shituleni SA, Huang K*. Effects of Selenium-Enriched Probiotics on Heat Shock Protein mRNA Levels in Piglet under Heat Stress Conditions. J Agric Food Chem. 2013, 61(10):2385-91.
40．Du L, Li B, He K*, Zhang H, Huang K*, Xiao S. Construction and immunogenicity of DNA vaccines encoding fusion protein of porcine IFN- λ 1 and GP5 gene of porcine reproductive and respiratory syndrome virus. Biomed Res Int. 2013:318698.
41．Chen X, Ren F, Hesketh J, Shi X, Li J, Gan F, Hu Z, Huang K*. Interaction of porcine circovirus type 2 replication with intracellular redox status in vitro. Redox Rep. 2013, 18(5):186-92.
42．Gan F, Chen X, Liao SF, Lv C, Ren F, Ye G, Pan C, Huang D, Shi J, Shi X, Zhou H, Huang K*. Selenium-enriched probiotics improve antioxidant status, immune function, and selenoprotein gene expression of piglets raised under high ambient temperature. J Agric Food Chem. 2014, 62(20):4502-8.
43．Ye G, Zhu Y, Liu J, Chen X, Huang K*. Preparation of glycerol-enriched yeast culture and its effect on blood metabolites and ruminal fermentation in goats. PLoS One. 2014, 9(4):e94410.
44．Parveen F, Nizamani ZA, Gan F, Chen X, Shi X, Kumbhar S, Zeb A, Huang K*. Protective effect of selenomethionine on aflatoxin B1-induced oxidative stress in MDCK cells. Biol Trace Elem Res. 2014 ,157(3):266-74.
45．Liu J, Ye G, Zhou Y, Liu Y, Zhao L, Liu Y, Chen X, Huang D, Liao SF, Huang K*. Feeding glycerol-enriched yeast culture improves performance, energy status, and heat shock protein gene expression of lactating Holstein cows under heat stress. J Anim Sci. 2014, 92(6):2494-502.
46．Gan F, Zhang Z, Hu Z, Hesketh J, Xue H, Chen X, Hao S, Huang Y, Cole Ezea P, Parveen F, Huang K*. Ochratoxin A promotes porcine circovirus type 2 replication in vitro and in vivo. Free Radic Biol Med. 2015, 80, 33–47.
47．Zhuang T, Xu H, Hao S, Ren F, Chen X, Pan C, Huang K*. Effects of selenium on proliferation, interleukin-2 production and selenoprotein mRNA expression of normal and dexamethasone-treated porcine splenocytes. Res Vet Sci. 2015, 98, 59–65
48．Liu Y, Liu Q, Ye G, Khan A, Liu J, Gan F, Zhang X, Kumbhar S, Huang K*. Protective Effects of Selenium-Enriched Probiotics on Carbon Tetrachloride-Induced Liver Fibrosis in Rats. J Agric Food Chem. 2014 Dec 31. [Epub ahead of print]
49．Du L, Li B, Xu X, Sun B, Pang F, Wen L, Huang K, He K. Adsorption of a porcine reproductive and respiratory syndrome virus DNA vaccine candidate onto biodegradable nanoparticles improves immunogenicity in mice. Arch Virol. 2015，160(6):1543-7. doi: 10.1007/s00705-015-2396-0.
50．Gan F, Xue H, Huang Y, Pan C, Huang K*. Selenium alleviates porcine nephrotoxicity of ochratoxin A by improving selenoenzyme expression in vitro. PLoS One. 2015，24，10(3):e0119808. doi: 10.1371/journal.pone.0119808.
51．Hao S, Pan S, Hu J, Qian G, Gan F, Huang K*. Aflatoxin B1 suppressed t-cell response to anti-pig-cd3 monoclonal antibody stimulation in primary porcine splenocytes: a role for the extracellular regulated protein kinase (ERK1/2) MAPK signaling pathway. J Agric Food Chem. 2015 Jun 24. [Epub ahead of print]
52．Liu Y, Xu H, Zhong W, Shen Q, Zhuang T, Huang K*. Organic selenium alleviated the formation of ethylene glycol-induced calcium oxalate renal calculi by improving osteopontin expression and antioxidant capability in dogs. Biol Trace Elem Res. 2015 May 28. [Epub ahead of print]
53．Chen X, Shi X, Gan F, Huang D, Huang K*. Glutamine starvation enhances PCV2 replication via the phosphorylation of p38 MAPK, as promoted by reducing glutathione levels. Vet Res. 2015 Mar 18;46:32. doi: 10.1186/s13567-015-0168-1.
54．Lv CH, Wang T, Regmi N, Chen X, Huang K*, Liao SF*. Effects of dietary supplementation of selenium-enriched probiotics on production performance and intestinal microbiota of weanling piglets raised under high ambient temperature. J Anim Physiol Anim Nutr (Berl). 2015 Dec; 99(6):1161-71. doi: 10.1111/jpn.12326. Epub 2015 Apr 21.
55．Xue H, Gan F, Zhang Z, Hu J, Chen X, Huang K*. Astragalus polysaccharides inhibits PCV2 replication by inhibiting oxidative stress and blocking NF-κB pathway. Int J Biol Macromol. 2015 Jul 29; 81:22-30. doi: 10.1016/j.ijbiomac.2015.07.050. [Epub ahead of print]
56．Nido SA, Shituleni SA, Mengistu BM, Liu Y, Khan AZ, Gan F, Kumbhar S, Huang K*. Effects of Selenium-Enriched Probiotics on Lipid Metabolism, Antioxidative Status, Histopathological Lesions, and Related Gene Expression in Mice Fed a High-Fat Diet. Biol Trace Elem Res. 2016 Jun;171(2):399-409. doi: 10.1007/s12011-015-0552-8. Epub 2015 Nov 6.
57．Xue J, Jiang W, Chen Y, Liu Y, Zhang H, Xiao Y, Qiao Y, Huang K*, Wang Q*. Twenty-six circulating antigens and two novel diagnostic candidate molecules identified in the serum of canines with experimental acute toxoplasmosis. Parasit Vectors. 2016 Jun 29;9(1):374. doi: 10.1186/s13071-016-1643-x
58．Wu C, Xu Z, Gai R, Huang K*. Matrine ameliorates spontaneously developed colitis in interleukin-10-deficient mice. Int Immunopharmacol. 2016 Jul;36:256-62. doi: 10.1016/j.intimp.2016.04.038. Epub 2016 May 12.
59．Wu C, Xu Z, Huang K*. Effects of Dietary Selenium on Inflammation and Hydrogen Sulfide in the Gastrointestinal Tract in Chickens. Biol Trace Elem Res. 2016 Dec;174(2):428-435. Epub 2016 May 13.
60．Wu C, Sartor RB, Huang K*, Tonkonogy SL*. Transient activation of mucosal effector immune responses by resident intestinal bacteria in normal hosts is regulated by interleukin-10 signalling. Immunology. 2016 Jul;148(3):304-14. doi: 10.1111/imm.12612.
61．Gan F, Hu Z, Huang Y, Xue H, Huang D, Qian G, Hu J, Chen X, Wang T, Huang K*. Overexpression of pig selenoprotein S blocks OTA-induced promotion of PCV2 replication by inhibiting oxidative stress and p38 phosphorylation in PK15 cells. Oncotarget. 2016 Apr 12;7(15):20469-85. doi: 10.18632/oncotarget.7814.
62． Qian G, Liu D, Hu J, Gan F, Hou L, Chen X, Huang K*. Ochratoxin A-induced autophagy in vitro and in vivo promotes porcine circovirus type 2 replication. Cell Death Dis. 2017 Jun 29;8(6):e2909. doi: 10.1038/cddis.2017.303.
63． Gan F, Hu Z, Zhou Y, Huang K*. Overexpression and Low Expression of Selenoprotein S Impact Ochratoxin A-Induced Porcine Cytotoxicity and Apoptosis in Vitro. J Agric Food Chem. 2017 Aug 16;65(32):6972-6981. doi: 10.1021/acs.jafc.7b02115. Epub 2017 Aug 7.
64． Gan F, Zhou Y, Hou L, Qian G, Chen X, Huang K*. Ochratoxin A induces nephrotoxicity and immunotoxicity through different MAPK signaling pathways in PK15 cells and porcine primary splenocytes.Chemosphere. 2017 Sep;182:630-637. doi: 10.1016/j.chemosphere.2017.05.030. Epub 2017 May 9.
65． Hamid M, Liu D, Abdulrahim Y, Liu Y, Qian G, Khan A, Gan F, Huang K*. Amelioration of CCl4-induced liver injury in rats by selenizing Astragalus polysaccharides: Role of proinflammatory cytokines, oxidative stress and hepatic stellate cells. Res Vet Sci. 2017 May 2;114:202-211. doi: 10.1016/j.rvsc.2017.05.002. [Epub ahead of print]
66． Wang H, Zhai N, Chen Y, Xu H, Huang K*. Cadmium induces Ca2+ mediated, calpain-1/caspase-3-dependent apoptosis in primary cultured rat proximal tubular cells.J Inorg Biochem. 2017 Jul;172:16-22. doi: 10.1016/j.jinorgbio.2017.04.005. Epub 2017 Apr 8.
67． Chen X, Che C, Korolchuk VI, Gan F, Pan C, Huang K*. Selenomethionine Alleviates AFB1-Induced Damage in Primary Chicken Hepatocytes by Inhibiting CYP450 1A5 Expression via Upregulated SelW Expression.J Agric Food Chem. 2017 Mar 29;65(12):2495-2502. doi: 10.1021/acs.jafc.6b05308. Epub 2017 Mar 21.
68． Hamid M, Liu D, Abdulrahim Y, Khan A, Qian G, Huang K*. Inactivation of Kupffer Cells by Selenizing Astragalus Polysaccharides Prevents CCl4-Induced Hepatocellular Necrosis in the Male Wistar Rat. Biol Trace Elem Res. 2017 Feb 27. doi: 10.1007/s12011-017-0970-x. [Epub ahead of print]
69． Xue H, Gan F, Qian G, Hu J, Hao S, Xu J, Chen X, Huang K*. Astragalus polysaccharides attenuate PCV2 infection by inhibiting endoplasmic reticulum stress in vivo and in vitro.Sci Rep. 2017 Jan 10;7:40440. doi: 10.1038/srep40440.
70． Liu D, Xu J, Qian G, Hamid M, Gan F, Chen X, Huang K*. Selenizing astragalus polysaccharide attenuates PCV2 replication promotion caused by oxidative stress through autophagy inhibition via PI3K/AKT activation. Int J Biol Macromol. 2018 Mar;108:350-359. doi: 10.1016/j.ijbiomac.2017.12.010. Epub 2017 Dec 5.
71． Gan F, Liu Q, Liu Y, Huang D, Pan C, Song S, Huang K*. Lycium barbarum polysaccharides improve CCl4-induced liver fibrosis, inflammatory response and TLRs/NF-kB signaling pathway expression in wistar rats. Life Sci. 2018 Jan 1;192:205-212. doi: 10.1016/j.lfs.2017.11.047. Epub 2017 Nov 28.
72． Wang H, Chen Y, Zhai N, Chen X, Gan F, Li H, Huang K*. Ochratoxin A-Induced Apoptosis of IPEC-J2 Cells through ROS-Mediated Mitochondrial Permeability Transition Pore Opening Pathway. J Agric Food Chem. 2017 Dec 6;65(48):10630-10637. doi: 10.1021/acs.jafc.7b04434. Epub 2017 Nov 20.
73． Zhou Y, Gan F, Hou L, Zhou X, Adam Ibrahim YA, Huang K*. Modulations of DNMT1 and HDAC1 are involved in the OTA-induced cytotoxicity and apoptosis in vitro. Chem Biol Interact. 2017 Dec 25;278:170-178. doi: 10.1016/j.cbi.2017.10.020. Epub 2017 Nov 5.
74． Khan AZ, Kumbhar S, Liu Y, Hamid M, Pan C, Nido SA, Parveen F, Huang K*. Dietary Supplementation of Selenium-Enriched Probiotics Enhances Meat Quality of Broiler Chickens (Gallus gallus domesticus) Raised Under High Ambient Temperature. Biol Trace Elem Res. 2018 Apr;182(2):328-338. doi: 10.1007/s12011-017-1094-z. Epub 2017 Jul 12.
75． Gan F, Hou L, Zhou Y, Liu Y, Huang D, Chen X, Huang K*. Effects of ochratoxin A on ER stress, MAPK signaling pathway and autophagy of kidney and spleen in pigs. Environ Toxicol. 2017 Oct;32(10):2277-2286. doi: 10.1002/tox.22443. Epub 2017 Jul 12.
76． Xue J, Jiang W, Chen Y, Gong F, Wang M, Zeng P, Xia C, Wang Q, Huang K*. Thioredoxin reductase from Toxoplasma gondii: an essential virulence effector with antioxidant function. FASEB J. 2017 Oct;31(10):4447-4457. doi: 10.1096/fj.201700008R. Epub 2017 Jul 7.
77． Kumbhar S, Khan AZ, Parveen F, Nizamani ZA, Siyal FA, El-Hack MEA, Gan F, Liu Y, Hamid M, Nido SA, Huang K*. Impacts of selenium and vitamin E supplementation on mRNA of heat shock proteins, selenoproteins and antioxidants in broilers exposed to high temperature. AMB Express. 2018 Jul 10;8(1):112. doi: 10.1186/s13568-018-0641-0.
78． Wang H, Zhai N, Chen Y, Fu C, Huang K*. OTA induces intestinal epithelial barrier dysfunction and tight junction disruption in IPEC-J2 cells through ROS/Ca2+-mediated MLCK activation. Environ Pollut. 2018 Jun 21;242(Pt A):106-112. doi: 10.1016/j.envpol.2018.06.062. [Epub ahead of print]
79． Liu Y, Liu Q, Hesketh J, Huang D, Gan F, Hao S, Tang S, Guo Y, Huang K*. Protective effects of selenium-glutathione-enriched probiotics on CCl4-induced liver fibrosis. J Nutr Biochem. 2018 May 1;58:138-149. doi: 10.1016/j.jnutbio.2018.04.011. [Epub ahead of print]
80． Sun Y, Liu J, Ye G, Gan F, Hamid M, Liao S, Huang K*. Protective effects of zymosan on heat stress-induced immunosuppression and apoptosis in dairy cows and peripheral blood mononuclear cells. Cell Stress Chaperones. 2018 Jun 2. doi: 10.1007/s12192-018-0916-z. [Epub ahead of print]
81． Gan F, Yang Y, Chen Y, Che C, Pan C, Huang K*. Bush sophora root polysaccharide could help prevent aflatoxin B1-induced hepatotoxicity in the primary chicken hepatocytes. Toxicon. 2018 Aug;150:180-187. doi: 10.1016/j.toxicon.2018.05.019. Epub 2018 May 29.
82． Hou L, Zhou X, Gan F, Liu Z, Zhou Y, Qian G, Huang K*. Combination of Selenomethionine and N-Acetylcysteine Alleviates the Joint Toxicities of Aflatoxin B1 and Ochratoxin A by ERK MAPK Signal Pathway in Porcine Alveolar Macrophages. J Agric Food Chem. 2018 Jun 13;66(23):5913-5923. doi: 10.1021/acs.jafc.8b01858. Epub 2018 Jun 1.
83． Gan F, Zhou Y, Qian G, Huang D, Hou L, Liu D, Chen X, Wang T, Jiang P, Lei X, Huang K*. PCV2 infection aggravates ochratoxin A-induced nephrotoxicity via autophagy involving p38 signaling pathway in vivo and in vitro. Environ Pollut. 2018 Jul;238:656-662. doi: 10.1016/j.envpol.2018.03.032. Epub 2018 Mar 31.
84． Hou L, Gan F, Zhou X, Zhou Y, Qian G, Liu Z, Huang K*. Immunotoxicity of ochratoxin A and aflatoxin B1 in combination is associated with the nuclear factor kappa B signaling pathway in 3D4/21 cells. Chemosphere. 2018 May;199:718-727. doi: 10.1016/j.chemosphere.2018.02.009. Epub 2018 Feb 3.
85． Xu J, Pan S, Gan F, Hao S, Liu D, Xu H, Huang K*. Selenium deficiency aggravates T-2 toxin-induced injury of primary neonatal rat cardiomyocytes through ER stress. Chem Biol Interact. 2018 Apr 1;285:96-105. doi: 10.1016/j.cbi.2018.01.021. Epub 2018 Feb 16.
86． Qian G, Liu D, Hu J, Gan F, Hou L, Zhai N, Chen X, Huang K*. SeMet attenuates OTA-induced PCV2 replication promotion by inhibiting autophagy by activating the AKT/mTOR signaling pathway. Vet Res. 2018 Feb 13;49(1):15. doi: 10.1186/s13567-018-0508-z.
87． Hamid M, Abdulrahim Y, Liu D, Qian G, Khan A, Huang K*. The Hepatoprotective Effect of Selenium-Enriched Yeast and Gum Arabic Combination on Carbon Tetrachloride-Induced Chronic Liver Injury in Rats. J Food Sci. 2018 Feb;83(2):525-534. doi: 10.1111/1750-3841.14030. Epub 2018 Jan 19.
