Yongjie Liu

Updated: 2018-07-23    Read:

               Basic Information    


Teacher Name: Yongjie Liu

Job Title: professor



AddressNo. 1 Weigang Road, College of Veterinary Medicine,

Nanjing Agricultural University, Nanjing 210095, China

Research Areas and Descriptors

Pathogenic mechanism of bacterial pathogens such as Aeromonas hydrophila and Streptococcus agalactiae, and interspecies transmission of canine influenza virus




09/1997-06/2000   Ph.D in Veterinary Medicine, Northeast Agricultural University, Harbin, China 

09/1994-06/1997   M.S in Veterinary Microbiology and Immunology, Jilin Agricultural University, Changchun, China 

09/1990-06/1994    B.S in Veterinary Medicine, Jilin Agricultural University, Changchun, China


12/2007-Present   Professor in College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China

01/2003-11/2007   Associate professor in College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China

10/2000-12/2002   Post-doctoral fellow in College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China

07/2005-10/2005   Visiting scholar in Universitaet Erlangen-Nuernberg, Germany

11/2006-02/2007   Visiting scholar in Universitaet Erlangen-Nuernberg, Germany


1.   Wang NNLiu JPang MDWu YFAwan FLiles MRLu CP, Liu YJ*. Diverse roles of Hcp family proteins in the environmental fitness and pathogenicity of Aeromonas hydrophila Chinese epidemic strain NJ-35. Appl Microbiol Biotechnol, 2018, 102: 7083–7095.

2.   Ma K, Cao Q, Luo S, Wang ZF, Liu GJ, Lu CP, Liu YJ*. Cas9 enhances bacterial virulence by repressing the regR transcriptional regulator in Streptococcus agalactiae. Infect Immun, 2018, 86(3): e00552-17.

3.   Liu JDong YHWang NNLi SGYang YYWang YAwan FLu CPLiu YJ*. Tetrahymena thermophila predation enhances environmental adaptation of the carp pathogenic strain Aeromonas hydrophila NJ-35. Front Cell Infect Microbiol, 2018, 8:76.

4.   Xie XNa WKang AYeom MYuk HMoon HKim SJKim HWKim JKPang MWang Y, Liu Y*Song D*. Comparison of the virulence and transmissibility of canine H3N2 influenza viruses and characterization of their canine adaptation factors. BMC Vet Res, 2018,14: 149.

5.   Awan F, Dong YH, Wang NN, Liu J, Ma K, Liu YJ*. The fight for invincibility: Environmental stress response mechanisms and Aeromonas hydrophila. Microb Pathog, 2018, 116:135-145.

6.   Pang MD, Xie X, Dong YH, Du HC, Wang NN, Lu CP, Liu YJ*. Identification of novel virulence-related genes in Aeromonas hydrophila by screening transposon mutants in a Tetrahymena infection model. Vet Microbiol, 2017, 199: 36-46.  

7.   Luo S, Cao Q, Ma K, Wang ZF, Liu GJ, Lu CP, Liu YJ*. Quantitative assessment of the blood-brain barrier opening caused by Streptococcus agalactiae hyaluronidase in a BALB/c mouse model. Sci Rep2017, 7(1):13529.

8.   Dong YH, Liu J, Pang MD, Du HC, Wang NN, Awan F, Lu CP, Liu YJ*. Catecholamine-stimulated growth of Aeromonas hydrophila requires the TonB2 energy transduction system but is independent of the amonabactin siderophore. Front Cell Infect Microbiol, 2016, 6:183.

9.   Pang MD, Lin XQ, Liu J, Guo CM, Gao SS, Du HC, Lu CP, Liu YJ*. Identification of Aeromonas hydrophila genes preferentially expressed after phagocytosis by Tetrahymena and involvement of methionine sulfoxide reductases. Front Cell Infect Microbiol, 2016, 6:199.

10.   Du HC, Pang MD, Dong YH, Wu YF, Wang NN, Liu J, Awan F, Lu CP, Liu YJ*. Identication and characterization of an Aeromonas hydrophila oligopeptidase gene pepF negatively related to biolm formation. Front Microbiol, 2016 7:1497.

11.   Lin Y, Xie X, Zhao YB, Kalhoro DH, Lu CP, Liu YJ*. Enhanced replication of avian-origin H3N2 canine influenza virus in cell culture and mice by a two-amino acid insertion in neuraminidase stalk.Vet Res, 2016, 47: 53

12.   Kalhoro DH, Gao SS, Xie X, Liang S, Luo S, Zhao YB, Liu YJ*.Canine influenza virus coinfection with Staphylococcus pseudintermedius enhances bacterial colonization, virus load and clinical presentation. BMC Vet Res, 2016,12: 87

13.   Xie X, Lin Y, Pang MD, Zhao YB, Kalhoro DH, Lu CP, Liu YJ*. Monoclonal antibody specific to HA2 glycopeptide protects mice from H3N2 influenza virus infection. Vet Res, 2015, 46: 33

14.   Xie X, Pang MD, Liang S, Yu L, Zhao YB, Ma K, Kalhoro DH, Lu CP, Liu YJ*.Establishment and characterization of a telomerase-immortalized canine bronchiolar epithelial cell line. Appl Microbiol Biotechnol, 2015,99(1):9135-9146

15.   Jiang W, Liu YC, Chen YJ, Yang QF, Chun P, Yao KL, Han XG, Wang SH, Yu SQ, Liu YJ*, QuanWang*. Novel dynamic flow immunochromatographic test (DFICT) using gold nanoparticles for the serological detection of Toxoplasma gondii infection in dogs and cats. Biosens Bioelectron, 2015, 72: 133–139

16.   Pang MD, Jiang JW, Xie X, Wu YF, Dong YH, Kwok A HY, Zhang W, Yao HC, Lu CP, Leung FC*, Liu YJ*. Novel insights into the pathogenicity of epidemic Aeromonas hydrophila ST251 clones from comparative genomics. Sci Rep, 2015, 5: 09833.

17.   Wang NN, Wu YF, Pang MD, Liu J, Liu YJ*, Lu CP. Protective efcacy of recombinant hemolysin co-regulated protein (Hcp) of Aeromonas hydrophila in Common carp (Cyprinus carpio). Fish Shell Immunol, 2015, 46(2):297-304.

18.   Cao DL, Wang NN, Lu CP, Liu YJ*. Identification and characterization of a novel virulence-associated metalloprotease from Aeromonas hydrophila. Pak Vet J, 2015, 35: 38-42.

19.   Guo P, Wang N, Liu YJ*, Lu CP. Antimicrobial susceptibility and characterization of outer membrane proteins of Aeromonas hydrophila isolated in China. J Integrative Agri, 2014, 13(4): 911-917.

20.   Jiang W, Han XG, Wang Q, Li XT, Yi L, Liu YJ*, Ding C*. Vibrio parahaemolyticus enolase is an adhesion-related factor that binds plasminogen and functions as a protective antigen. Appl Microbiol Biotechnol, 2014, 98:4937–4948.

21.   Wang ZF, Guo CM, Xu YN, Liu GJ, Lu CP, Liu YJ*. Two novel functions of hyaluronidase from Streptococcus agalactiae are enhanced intracellular survival and inhibition of proinammatory cytokine expression. Infect Immun, 2014, 82: 2615-2625.

22.   Guo CM, Chen RR, Kalhoro DH, Wang ZF, Liu GJ, Lu CP, Liu YJ*. Identification of genes preferentially expressed by highly virulent piscine Streptococcus agalactiae upon interaction with macrophages. PLoS ONE, 2014, 9: e87980.

23.  Wang N, Yang Z, Zang MF, Liu YJ*, Lu CP. Identification of Omp38 by immunoproteomic  analysis and evaluation as a potential vaccine antigen against Aeromonas hydrophila in Chinese  breams. Fish Shell Immunol, 2013, 34: 74-81.

24.  Zeng XJ, Lin Y, Zhao YB, Lu CP, Liu YJ*. Experimental infection of H3N2 canine influenza virus from China. Epidemiol Infect, 2013, 141(12): 2595-2603.

25.   Lin Y, Zhao YB, Zeng XJ, Lu CP, Liu YJ*. Genetic and pathobiologic characterization of H3N2 canine influenza viruses isolated in the Jiangsu Province of China in 2009-2010. Vet Microbiol, 2012, 158: 247- 258.

26.   Pang MD, Lin XQ, Hu M, Li J, Lu CP, Liu YJ*. Tetrahymena: an alternative model host for evaluating virulence of Aeromonas strains. PLoS ONE2012, 7 (11): e48922.

27.   Hu M, Wang N, Pan ZH, Lu CP, Liu YJ*. Identity and virulence properties of Aeromonas isolates from diseased fish, healthy controls, and water environment in China. Lett in Appl Microbiol, 2012, 55: 224-233.

28.   Li J, Zhang XL, Liu YJ*, Lu CP. Development of an Aeromonas hydrophila infection model using the protozoan Tetrahymena thermophila. FEMS Microbiol Lett, 2011, 316: 160-168.

29.   Li J, Ni XD, Liu YJ*, Lu CP. Detection of three virulence genes alt, ahp and aerA in Aeromonas hydrophila and their relationship with actual virulence to zebrash. J Appl Microbiol, 2011, 110: 823–830.

30.   Ni XD, Wang N, Liu YJ*, Lu CP. Immunoproteomics of extracellular proteins of Aeromonas hydrophila China vaccine strain J-1 reveal a highly immunoreactive outer membrane protein. FEMS Immunol Med Microbiol, 2010, 58 (3): 363-373.

GRANTS (Principal applicant)

1.Studies on broad-spectrum neutralizing antibodies against major animal diseases. National key research and development plan (2017YFD0501101), 01/2017-12/2020

2.Study on the prevention and control of bacterial septicemia in Carassius auratus. Independent Innovation Fund of Agricultural Science and Technology in Jiangsu Province (CX(17)2027), 04/2017-6/2020

3.Preliminary study on etiology of hepatopancreas lesion syndrome and integration and demonstration of comprehensive prevention and control technology. Aquatic Three New Projects in Jiangsu Province (D2017-3-1). 04/2017-6/2020

4.Pathobiology and interspecies transmission of canine influenza virus isolates from China and South Korea. International S&T Cooperation Program of China (ISTCP 2014DFG32770), 04/2014-12/2017

5.Virulence evolution of Aeromonas hydrophila mediated by Tetrahymena and molecular mechanisms. National Natural Science Foundation of China (31372454), 01/2014-12/2018

6. Investigation of main bacterial infections in fish in Jiangsu Province. Aquatic Three New Projects in Jiangsu Province(D2013-5-4), 07/2013-12/2015

7. Construction of virulence evaluation model using Tetrahymena for high-throughput screening of Aeromonas hydrophila virulence-associated genes. National Natural Science Foundation of China (31072151), 01/2011-12/2013

8. Characterization of antigenic proteins of Aeromonas hydrophila during growth as a biofilm. Specialized Research Fund for the Doctoral Program of Higher Education of China (20090097110007), 01/2010-12/2012

9. Program for New Century Excellent Talents in University (NCET-07-0440), 01/2008-12/2010

10. Comprehensive prevention and control of hemorrhage disease in freshwater fish. Special Funding of Public Sector Agricultural Research Project from the Chinese Ministry of Agriculture (200803013), 01/2008-12/2010

11. Display of bacterial antigens by ciliates surface. Jiangsu Provincial Natural Science Foundation of China (BK2007155), 01/2007-12/2009

12. Identification of type III secretion system in Aeromonas hydrophila J-1 and its potential use as a vaccine candidate. International Foundation for Science (A/4108-1)01/2006-12/2008

13. Design of bacterial genetic vaccine using protozoan as expression and carrier. National Natural Science Foundation of China (No. 30400332), 01/2005-12/2007

14. Study on pathogenicity island of Aeromonas hydrophila. Key Item of Educational Ministry of China (105091), 01/2005-12/2007