Basic Information
Teacher Name: Xiaokai Song
Job Title: Professor
Phone: 86-25-84395904
Email：songxiaokai@njau.edu.cn
Address：Room 4015 in Yifu Building
Research Areas and Descriptors：Veterinary parasitology
and immunity, veterinary public health
Profile
Ph.D., Professor. His research areas are molecular immunity of veterinary parasites and veterinary public health. He is a member of a council of veterinary parasitology branch and veterinary food hygiene branch of Chinese Association of Animal Science and Veterinary Medicine. He is a member of the youth committee of commission on parasitology of the China Zoological Society. He graduated and obtained his master's degree from Henan Agricultural University in 2002.He graduated and obtained his doctor's degree from Nanjing Agricultural University in 2008. After graduation, he was employed as a teacher in Nanjing Agricultural University in 2008. He was employed as an associate professor and professor successively in 2010 and 2016. From 2014 to 2015, he worked in Aarhus University in Denmark as a visiting scholar. As principal, he undertakes 9 scientific research projects, including 1 sub-project of the National Key R&D Program of China, 1 sub-project of the “National Key Basic Research Program (973 Program) of China, 2 project of the National Natural Science Foundation of China, 2 project of the Natural Science Foundation of Jiangsu Province of China,1 project of the Fundamental Research Funds for the Central Universities et al.As the main participant, he undertakes3 research projects.He published more than 100 articles. He took part in writing 5 books. As the main participant, he obtained 7 patents. He is one of the main teachers of the course of Veterinary Parasitologywhich has been awarded as the National Excellent Course and the National Resource Sharing Course.
Main articles (* Corresponding author)
1. 刘剑华，刘连瑞，李灵娟，徐立新，严若峰，李祥瑞，宋小凯*. 鸡球虫共同抗原UCE免疫原性分析及其对多种球虫感染的免疫保护效果评估. 南京农业大学学报 2018，41(3): 496-503.
2. Tian L, Li W, Huang X, Tian D, Liu J, Yang X, Liu L, Yan R, Xu L, Li X, Song X*.Protective Efficacy of Coccidial Common Antigen Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) against Challenge with ThreeEimeriaSpecies.Front Microbiol. 2017, 8:1245.
3. Liu L, Huang X, Liu J, Li W, Ji Y, Tian D, Tian L, Yang X, Xu L, Yan R, Li X, Song X*.Identification of common immunodominant antigens of Eimeria tenella, Eimeria acervulina and Eimeria maxima by immunoproteomic analysis.Oncotarget. 2017, 8(21):34935-34945.
4. Yang X, Li M, Liu J, Ji Y, Li X, Xu L, Yan R, Song X*. Identification of immune protective genes of Eimeria maxima through cDNA expression library screening. Parasite Vectors 2017, 10(1):85.
5. Song X, Zhao X, Xu L, Yan R, Li X. Immune protection duration and efficacy stability of DNA vaccine encoding Eimeria tenella TA4 and chicken IL-2 against coccidiosis. Res Vet Sci. 2016, 111:31-35.
6. Song X, Zhang Z, Liu C, Xu L, Yan R, Li X. Evaluation of the persistence, integration, histopathology and environmental release of DNA vaccine encoding Eimeria tenella TA4 and chicken IL-2. Vet Parasitol. 2016, 229: 22-30.
7. Song X, Gao Y, Xu L, Yan R, Li X. Partial protection against four species of chicken coccidia induced by multivalent subunit vaccine. Vet Parasitol. 2015 212(3-4):80-85.
8. Song X, Zhang R, Xu L, Yan R, Li X. Chimeric DNA vaccines encoding Eimeria acervulina macrophage migration inhibitory factor (E.MIF) induce partial protection against experimental Eimeria infection. ActaParasitol. 2015 60(3):500-8.
9. Song X,Xu L, Yan R, Huang X, Li X. Construction of Eimeria tenella multi-epitope DNA vaccines and their protective efficacies against experimental infection. Vet ImmunolImmunopathol. 2015 166(3-4):79-87.
10. Song X, Huang X, Yan R, Xu L, Li X. Efficacy of chimeric DNA vaccines encoding Eimeria tenella 5401 and chicken IFN-γ or IL-2 against coccidiosis in chickens. ExpParasitol. 2015 156:19-25.
11. Song X, Ren Z, Yan R, Xu L, Li X. Induction of protective immunity against Eimeria tenella, Eimeria necatrix, Eimeria maxima and Eimeria acervulina infections using multivalent epitope DNA vaccines. Vaccine. 2015 33(24):2764-70.
12. 李梦辉，刘连瑞，涂浩，黄经纬，张振超，徐立新，严若峰，李祥瑞，宋小凯*.利用gateway 技术构建巨型艾美耳球虫孢子化卵囊cDNA表达文库. 南京农业大学学报 2015,38（4）：630-635.
13. 朱艳，滕佩，魏德康，张振超，徐立新，严若峰，李祥瑞，宋小凯*. 柔嫩艾美耳球虫江苏株微线蛋白-3 基因的克隆与序列分析. 畜牧与兽医 2014， 46（8）：75-79.
14. Xiaokai Song, Lixin Xu, Ruofeng Yan, Xinmei Huang, Mohammed Ali A. Shah, Xiangrui Li*. The optimal immunization procedure of DNA vaccine pcDNA-TA4-IL-2 of Eimeria tenella, its cross immunity to Eimeria necatrix and Eimeria acervulina. Veterinary parasitology (IF: 2.579), 2009，159(1)：30-36.
15. Qianming Xu, Xiaokai Song(并列第一作者), Lixin Xu, Ruofeng Yan, Mohammed Ali A. Shah, Xiangrui Li.Vaccination of chickens with a chimeric DNA vaccine encoding Eimeria tenella TA4 and chicken IL-2 induces protective immunity against coccidiosis. Veterinary Parasitology,2008,156(3-4): 319-323.
16. Sun W, Song X, Yan R, Xu L, Li X. Vaccination of goats with a glutathione peroxidase DNA vaccine induced partial protection against Haemonchus contortus infection. Vet Parasitol 2011, 182(2-4): 239-247.
17. Muhammad Ali A. Shah, Xiaokai Song, LixinXu, Ruofeng Yan, Xiangrui Li*, Construction of DNA vaccines encoding Eimeria acervulina cSZ-2 with chicken IL-2 and IFN-c and their efficacy against poultry coccidiosis. Research in Veterinary Science, 2011, 90: 72–77
18. Wei Sun, Xiaokai Song, Ruofeng Yan, LixinXu, Xiangrui Li. Cloning and characterization of a selenium-independent glutathione peroxidase (HC29) from adult Haemonchus contortus. J Vet Sci, 2012, 13(1), 49-58.
19. Tang F, Xu L, Yan R, Song X and Li X. Evaluation of the immune response induced by DNA vaccines expressing MIF and MCD1genes of Trichinellaspiralis in BALB/c mice. J Helminthol. 2012, 86:430–439
20. Kaikai Han, LixinXu, Ruofeng Yan, Xiaokai Song, Xiangrui Li*. Molecular cloning, expression and characterization of enolase from adult Haemonchus contortus. Research in Veterinary Science, 2012, 92: 259–265
21. GuangWei Zhao, Bo Shen, Qing Xie, Li XinXu, RuoFeng Yan, Xiao Kai Song, I.A. Hassan, Xiang Rui Li∗. Detection of Toxoplasma gondii in free-range chickens in China based on circulating antigens and antibodies. Veterinary Parasitology, 2012, 18, 572–577
21. Han K, Xu L, Yan R, Song X, Li X*. Cloning, expression and characterization of NAD1-dependent glyceraldehyde-3-phosphate dehydrogenase of adult Haemonchus contortus. Journal of Helminthology, 2011, 85: 421–429
22. Han K, Xu L, Yan R, Song X, Li X. Vaccination of goats with glyceraldehyde-3-phosphate dehydrogenase DNA vaccine induced partial protection against Haemonchus contortus. Vet ImmunolImmunopathol. 2012, 149: 177-185
23. Zhao G, Shen B, Xie Q, Xu L, Yan R, Song X, Hassan Ibrahim Adam and Li X. Isolation and Molecular Characterization of Toxoplasma gondii from Chickens in China. Journal of Integrative Agriculture, 2012, 11(8): 1347-1353
24. Huili Zhu, Ruofeng Yan, Song Wang, Xiaokai Song, LixinXu, Xiangrui Li. Identification and molecular characterization of a novel antigen of Eimeria acervulina. Molecular and Biochemical Parasitology, 2012, 186: 21-28
25. Zhu H, Xu L, Yan R, Song X, Tang F, Wang S, Li X. Identification and characterization of a cDNA clone-encoding antigen of Eimeria acervulina. Parasitology, 2012, 139: 1711–1719.
26. Tang F, Xu L, Yan R, Song X, Li X. A DNA vaccine co-expressing Trichinellaspiralis MIF and MCD-1 with murine ubiquitin induces partial protective immunity in mice. J Helminthol. 2013, 87:24-33
27. Hongyan Song, BaofengQiu, Ruofeng Yan, LixinXu, Xiaokai Song, Xiangrui Li . The protective efficacy of chimeric SO7/IL-2 DNA vaccine against coccidiosis in chickens. Research in Veterinary Science. 2013, 94: 562–567.
28. Ruofeng Yan, Wei Sun, Xiaokai Song, LixinXu, XiangruiLi.Vaccination of goats with DNA vaccine encoding Dim-1 induced partial protection against Haemonchus contortus: A preliminary experimental study. Res. Vet. Sci. 2013, 95:189–199.
29. Ruofeng Yan, LixinXu, Jingjing Wang, Xiaokai Song, Xiangrui Li. Cloning and characterization of aldolase from parasitic nematode Haemonchus contortus. Journal of Animal and Veterinary Advances, 2013,12(4):478-486.
30. Meng Zhang, Zhen Yang, Shuai Wang, LongFei Tao, LiXinXu, RuoFeng Yan, XiaoKai Song, XiangRui Li. Detection of Toxoplasma gondii in shellfish and fish in parts of China. Veterinary Parasitology, 2014, 200, 1-2: 85-89.
31. Shuai Wang, GuangWei Zhao, Wang Wang, Qing Xie, Meng Zhang, Cheng Yuan, Ibrahim Adam Hassan, XinChao Liu, LiXinXu, RuoFeng Yan, XiaoKai Song, and XiangRui Li. Pathogenicity of two Toxoplasma gondii strains in chickens of different ages infected via intraperitoneal injection. Avian Pathology, 2014, 43, 1: 1–5.
32. Wang Wang, Cheng Yuan, Shuai Wang, XiaoKai Song, LiXinXu, RuoFeng Yan, I.A. Hasson, XiangRui Li. Transcriptional and proteomic analysis reveal recombinant galectins of Haemonchus contortus down-regulated functions of goat PBMC and modulation of several signaling cascades in vitro. Journal of Proteomics, 2014, 98, 26:123–137.
33. Tran DucHoan, Doan ThiThao , Javaid Ali Gadahi, Xiaokai Song, LixinXu, Ruofeng Yan, Xiangrui Li. Analysis of humoral immune response and cytokines in chickens vaccinated with Eimeria brunetti apical membrane antigen-1(EbAMA1) DNA vaccine. Experimental Parasitology. 2014,144: 65–72.
34. Wang Wang, Shuai Wang, Hui Zhang, Cheng Yuan, RuoFeng Yan, XiaoKai Song, LiXinXu and XiangRui Li. GalectinHco-gal-m from Haemonchus contortus modulates goat monocytes and T cell function in different patterns. Parasites & Vectors 2014, 7:342.
35. Ruofeng Yan, Jingjing Wang, LixinXu, Xiaokai Song and Xiangrui Li*. DNA vaccine encoding Haemonchus contortus Actin induces partial protection in Goats. ActaParasitologica, 2014, 59(4), 698–709.
36. Ibrahim A Hassan, Shuai Wang, LiXinXu, RuoFeng Yan, XiaoKai Song and Xiangrui Li. DNA vaccination with a gene encoding Toxoplasma gondiiDeoxyribose Phosphate Aldolase (TgDPA) induces partial protective immunity against lethal challenge in mice. Parasites & Vectors 2014, 7:431
37. Ibrahim A. Hassan, Shuai Wang, LiXinXu, RuoFeng Yan, XiaoKai Song,XiangRui Li. Immunoglobulin and cytokine changes induced following immunization with a DNA vaccine encoding Toxoplasma gondii selenium-dependent glutathione reductase protein. Experimental Parasitology, 2014, 146: 1–10
38. Hassan A, Wang S, Xu L, Yan R, Song X, Li X*. Immunological response and protection of mice immunized with plasmid encoding Toxoplasma gondii glycolytic enzyme malate dehydrogenase. Parasite Immunology, 2014, 36, 674–683
39. ZhenChao Zhang, JingWei Huang, MengHui Li, YuXia Sui, Shuai Wang, LianRui Liu, LiXinXu, RuoFeng Yan, XiaoKai Song, XiangRui Li*. Identification and molecular characterization of microneme 5 of Eimeria acervulina. PLoS One. 2014, 9(12):e115411.
40. Shuai Wang, Ibrahim A. Hassan1, XinChao Liu, LiXinXu, RuoFeng Yan, XiaoKai Song, XiangRui Li. Immunological changes induced by Toxoplasma gondii Glutathione-S-Transferase (TgGST) delivered as a DNA vaccine. Research in Veterinary Science, 2015, 99, 157–164
41. Cheng Yuan, Hui Zhang, Wang Wang, Yan Li, RuoFeng Yan, LiXinXu, XiaoKai Song and XiangRui Li*.Transmembrane protein 63A is a partner protein of Haemonchus contortus galectin in the regulation of goat peripheral blood mononuclear cells. Parasites & Vectors 2015, 8:211
42. Zhao Guangwei, Wang Shuai, Wang Wang, Zhang Zhenchao, Xie Qing, Zhang Meng, I A Hassan, Yan Ruofeng, Song Xiaokai,XuLixin, Li Xiangrui. Type I strain of Toxoplasma gondii from chicken induced different immune responses with that from human, cat and swine in chicken. Journal of Integrative Agriculture 2015, 14(5): 956–965
43. Wang S, Zhang M, Liu, Lin T, Yang H, Yuan S, Zhao G, HASSAN IA, Yan R, Song X, Xu L, Li X. Investigation on the co-infections of Toxoplasma gondii with PRRSV, CSFV or PCV-2 in swine in part of China. Journal of Integrative Agriculture, 2015, 14(9): 1838–1844
44. Huang Jingwei, Zhenchao Zhang, Menghui Li, Xiaokai Song, Ruofeng Yan, LixinXu, Xiangrui Li. Eimeria maxima microneme protein 2 delivered as DNA vaccine and recombinant protein induces immunity against experimental homogenous challenge. Parasitology International, 2015, 64: 408–416
45. Jingwei Huang, Zhenchao Zhang, Menghui Li, Xiaokai Song, Ruofeng Yan, LixinXu&Xiangrui Li. Immune protection of Microneme 7 (EmMIC7) against Eimeria maxima challenge in chickens. Avian Pathology, 2015, 44, (5): 392–400
46. Shuai Wang, Yujian Wang, Xiaoni Sun, Zhenchao Zhang, Tingqi Liu, Javaid Ali Gadahi, LixinXu, Ruofeng Yan, Xiaokai Song, Xiangrui Li. Protective immunity against acute toxoplasmosis in BALB/c mice induced by a DNA vaccine encoding Toxoplasma gondii 10 kDa excretory–secretory antigen (TgESA10). Veterinary Parasitology, 2015, 214: 40–48
47. Shuai Wang, YuJian Wang, XiaoNi Sun, ZhenChao Zhang, TingQi Liu, Javaid Ali Gadahi, Ibrahim Adam Hassan, LiXinXu, RuoFeng Yan, XiaoKai Song and XiangRui Li. Protective immunity against acute toxoplasmosis in BALB/c mice induced by a DNA vaccine encoding Toxoplasma gondii elongation factor 1-alpha. BMC Infectious Diseases (2015) 15:448
48. Shuai Wang, Guang-Wei Zhao, Wang Wang, Zhen-Chao Zhang, Bo Shen, I. A. Hassan, Qing Xie, Ruo-Feng Yan, Xiao-Kai Song, Li-XinXu, Xiang-Rui Li* Pathogenicity of Five Strains of Toxoplasma gondii from Different Animals to Chickens. Korean J Parasitol, 2015, 53(2): 155-162
49. ZhenChao Zhang, Shuai Wang, JingWei Huang, LianRui Liu, MingMin Lu, MengHui Li, YuXia Sui, LiXinXu, RuoFeng Yan, XiaoKai Song, XiangRui Li. ∗Proteomic analysis of Eimeria acervulina sporozoite proteins interaction with duodenal epithelial cells by shotgun LC–MS/MS. Molecular & Biochemical Parasitology, 2015, 202: 29–33
50. Ulrich-Lynge SL, Dalgaard TS, Norup LR, Song X, Sørensen P, Juul-Madsen HR. Chicken mannose-binding lectin function in relation to antibacterial activity towards Salmonella enterica. Immunobiology. 2015, 220(5):555-563
51. ZhenChao Zhang, LianRui Liu, JingWei Huang, Shuai Wang, MingMin Lu, XiaoKai Song, LiXinXu, RuoFeng Yan, XiangRui Li*. The molecular characterization and immune protection of microneme 2 of Eimeria acervulina. Veterinary Parasitology, 2016, 215: 96–105
52. Tran DucHoan, Zhenchao Zhang, Jingwei Huang, Ruofeng Yan, Xiaokai Song, LixinXu, Xiangrui Li*. Identification and immunogenicity of microneme protein 2 (EbMIC2) of Eimeria brunetti. Experimental Parasitology, 2016, 162: 7–17.
53. Wang F, Xu L, Song X, Li X, Yan R. Identification of differentially expressed proteins between free-living and activated third-stage larvae of Haemonchus contortus. Vet Parasitol., 2016, 215:72-77.
54. Zhang Z, Liu X, Yang X, Liu L, Wang S, Lu M, Ehsan M, Gadahi JA, Song X, Xu L, Yan R, Li X. The Molecular Characterization and Immunity Identification of Microneme 3 of Eimeria acervulina. J EukaryotMicrobiol., 2016, 63(6):709-721.
55. Gadahi JA, Yongqian B, Ehsan M, Zhang ZC, Wang S, Yan RF, Song XK, Xu LX, Li XR. Haemonchus contortus excretory and secretory proteins (HcESPs) suppress functions of goat PBMCs in vitro. Oncotarget. 2016, 7(24):35670-35679.
56. Gadahi JA, Li B, Ehsan M, Wang S, Zhang Z, Wang Y, Hasan MW, Yan R, Song X, Xu L, Li X. Recombinant Haemonchus contortus 24 kDa excretory/secretory protein (rHcES-24) modulate the immune functions of goat PBMCs in vitro. Oncotarget. 2016, 7(51):83926-83937.
57. Gadahi JA, Wang S, Bo G, Ehsan M, Yan R, Song X, Xu L, Li X. Proteomic Analysis of the Excretory and Secretory Proteins of Haemonchus contortus (HcESP) Binding to Goat PBMCs In Vivo Revealed Stage-Specific Binding Profiles. PLoS One 2016,11(7):e0159796.
58. Li Y, Yuan C, Wang L, Lu M, Wang Y, Wen Y, Yan R, Xu L, Song X, Li X. Transmembrane protein 147 (TMEM147): another partner protein of Haemonchus contortus galectin on the goat peripheral blood mononuclear cells (PBMC). ParasitVectors 2016, 9(1): 355.
59. Wang S, Zhang Z, Wang Y, Gadahi JA, Xu L, Yan R, Song X, Li X. Toxoplasma gondii Elongation Factor 1-Alpha (TgEF-1α) Is a Novel Vaccine Candidate Antigen against Toxoplasmosis. Front Microbiol. 2017, 8:168.
60. 严若峰,宋小凯,徐立新,李祥瑞. 基于ITS序列的捻转血矛线虫系统进化分析. 畜牧兽医学报,2012, 43(7):1117-1122
61. 严若峰,闫峰宾,王晶晶,徐立新,宋小凯,李祥瑞.捻转血矛线虫肌动蛋白基因的克隆与表达及其DNA 疫苗的构建.南京农业大学学报，2012,35(4):87-93
62. 董文阳,蒋静雅,陆浩,宋小凯,徐立新,严若峰,李祥瑞.南京地区流浪犬钩虫感染情况调查. 畜牧与兽医,2012,44(5):77-79
63. 张晓燕，严若峰，徐立新，宋小凯，李祥瑞.山羊白细胞介素-４成熟蛋白基因的原核表达及其生物学活性检测. 畜牧兽医学报,2012,43(10):1675-1663
64. 张志凯, 徐立新, 宋小凯, 李祥瑞, 严若峰. 捻转血矛线虫新基因Hcher-1 的克隆与特性分析. 南京农业大学学报, 2012,35(6):89-96
65. 牛延萍, 高丽丽, 宋小凯, 徐立新, 李祥瑞, 严若峰. 捻转血矛线虫ES24抗原基因的克隆与表达特性分析. 畜牧与兽医，2012,44（11）:17-20
66. 高波，袁橙，宋小凯，徐立新，严若峰，李祥瑞。捻转血矛线虫谷胱甘肽过氧化物酶体外对山羊外周血单核细胞功能的影响.畜牧与兽医，2014，46（5）：19-23
67. 张旭亮；张振超；苏苗苗；孙悦；李鹏飞；徐立新；宋小凯；李祥瑞；严若峰。旋毛虫醛缩酶基因的克隆表达及重组蛋白酶比活性的分析。中国兽医科学，2014，44（05）：497-502
68. 涂浩，赵星灿，杨占娜，李梦辉，徐立新，严若峰，宋小凯，李祥瑞. 猪重组IL-2、IL-4和IFN-γ对口蹄疫合成肽疫苗的免疫增强作用研究. 畜牧兽医学报 2015, 46(8): 1390-1399
