CURRICULUM VITAE
PERSONAL DETAILS
Name: Zongfu WU
Nationality : Chinese
Present position: Professor in College of Veterinary Medicine, Nanjing
Agricultural University
Address: No. 1 Weigang, College of Veterinary Medicine,
Nanjing Agricultural University, Nanjing 210095,
China
Tel: 0086-18651836283; 0086-25-84398606
Email: wuzongfu@njau.edu.cn
RESEARCH INTERESTS
My present research focuses on:
1) the identification and characterization of non-coding RNAs involved in Streptococcus suis virulence, as well as RNA-mediated regulations;
2) the characterization of new Streptococcus suis virulence factors identified by global approaches including deep sequencing, proteomics, and comparative genomic analysis.
EDUCATION
09/2006-06/2009 Ph.D, |
Veterinary microbiology and immunology, |
|
Nanjing Agricultural University, Nanjing, China |
09/2003-06/2006 |
M.S, |
Veterinary medicine, Fujian Agriculture and |
|
Forestry University, Fujian, China |
09/1999-06/2003 |
B.S, |
Animal science, Fujian Agriculture and Forestry |
|
University, Fujian, China |
PROFESSIONAL EXPERIENCE
01/2018-Present Professor in College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China
01/2012-12/2017 Associate professor in College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China
10/2014-10/2015 Visiting scholar in Dr. Pascale Romby’s lab, Architecture et Réactivité de l’ARN (UPR 9002 CNRS) , Institute of Molecular and Cellular Biology (IBMC), Strasbourg, France
Research: Identification of the functions of novel non-coding RNAs and their regulatory circuits in Staphylococcus aureus: their roles in stress adaptation and virulence
01/2010-02/2011 Post-doctoral fellow in Prof. Pascale Cossart’s lab, Bacteria-cell interactions Unit, Institut Pasteur, Paris, France.
Research: New small RNAs involved in Listeria monocytogenes virulence
07/2009-12/2011 Lecturer in College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China
09/2006-06/2009 Ph.D study supervised by Prof. Chengping Lu, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
Research: Screening virulence factors and immunogenic proteins of Streptococcus suis and transcriptome profiling of zebrafish due to Streptococcus suis infection
PUBLICATIONS (Corresponding author*)
1. Shouming Zhang, Xueping Gao, Genhui Xiao, Chengping Lu, Huochun Yao, Hongjie Fan, Zongfu Wu*. Intracranial Subarachnoidal Route of Infection for Investigating Roles of Streptococcus suis Biofilms in Meningitis in a Mouse Infection Model. Journal of Visualized Experiments, 2018 (137), e57658.
2. Liying Lai, Jiao Dai, Huanyu Tang, Shouming Zhang, Chunyan Wu, Wancen Qiu, Chengping Lu, Huochun Yao, Hongjie Fan, Zongfu Wu*. Streptococcus suis
serotype 9 strain GZ0565 contains a type VII secretion system putative substrate EsxA that contributes to bacterial virulence and a vanZ-like gene that confers resistance to teicoplanin and dalbavancin in Streptococcus agalactiae, Veterinary Microbiology, 2017, 205:26-33.
3. Genhui Xiao, Huanyu Tang, Shouming Zhang, Haiyan Ren, Jiao Dai, Liying Lai, Chengping Lu, Huochun Yao, Hongjie Fan, Zongfu Wu*. Streptococcus suis small RNA rss04 contributes to the induction of meningitis by regulating capsule synthesis and by inducing biofilm formation in a mouse infection model. Veterinary Microbiology, 2017, 199: 111-119.
4. Zongfu Wu, Jing Shao, Haiyan Ren, Huanyu Tang, Mingyao Zhou, Jiao Dai,
Liying Lai, Huochun Yao, Hongjie Fan, Dai Chen, Jie Zong, Chengping Lu. A Streptococcus suis LysM domain surface protein contributes to bacterial virulence. Veterinary Microbiology, 2016, 187: 64-69.
5. Zongfu Wu, Chunyan Wu, Jing Shao, Zhenzhen Zhu, Weixue Wang, Wenwei Zhang, Min Tang, Na Pei, Hongjie Fan, Jiguang Li, Huochun Yao, Hongwei Gu, Xun Xu, Chengping Lu. The Streptococcus suis transcriptional landscape reveals adaptation mechanisms in pig blood and cerebrospinal fluid. RNA, 2014, 20(6):882-898.
6. Zongfu Wu, Weixue Wang, Min Tang, Jing Shao, Chen Dai, Wei Zhang, Hongjie Fan, Huochun Yao, Jie Zong, Dai Chen, Junning Wang, Chengping Lu. Comparative genomic analysis shows that Streptococcus suis meningitis isolate SC070731 contains a unique 105K genomic island. Gene, 2014, 535(2):156-164.
7. Zongfu Wu, Wei Zhang, Yang Wang, Yan Lu, Chengping Lu. Identification of Antigens Common to Streptococcus suis Serotypes 2 and 9 by Immunoproteomic Analysis. Journal of Integrative Agriculture, 2012, 11(9):1517-1527.
8. Zongfu Wu, Wei Zhang, Jing Shao, Yang Wang, Yan Lu, Chengping Lu. Immunoproteomic assay of secreted proteins of Streptococcus suis serotype 9 with convalescent sera from pigs. Folia microbiologica, 2011, 56(5): 423-430.
9. Zongfu Wu, Wei Zhang, Yan Lu, ChengpingLu. Transcriptome profiling of zebrafish due to Streptococcus suis infection. Microbial Pathogenesis, 2010, 48(5):178-187.
10. Zongfu Wu, Wei Zhang, ChengpingLu. Comparative proteome analysis of secreted proteins of Streptococcus suis serotype 9 isolates from diseased and healthy pigs. Microbial Pathogenesis, 2008, 45(3): 159-166.
11. Zongfu Wu, Wei Zhang, ChengpingLu. Immunoproteomic assay of surface proteins of Streptococcus suis serotype 9. FEMS Immunology and Medical Microbiology, 2008, 53(1): 52-59.
12. Genhui Xiao, Zongfu Wu, Shouming Zhang, Huanyu Tang, Fengqiu Wang, Jiao Dai, Chengping Lu. Mac Protein is not an Essential Virulence Factor for the Virulent Reference Strain Streptococcus suis P1/7. Current Microbiology, 2017, 74
(1): 90-96.
13. Delphine Bronesky, Zongfu Wu, Stefano Marzi, Philippe Walter, Thomas Geissmann, Karen Moreau, François Vandenesch, Isabelle Caldelari, and Pascale Romby. Staphylococcus aureus RNAIII and Its Regulon Link Quorum Sensing, Stress Responses, Metabolic Adaptation, and Regulation of Virulence Gene Expression. Annual Review of Microbiology, 2016, 70: 299-316
14. Pierre Fechter, Delphine Parmentier, Zongfu Wu, Olivier Fuchsbauer, Pascale Romby, Stefano Marzi. Traditional Chemical Mapping of RNA Structure In Vitro and In Vivo. Methods in Molecular Biology, 2016, 1490: 83-103.
15. Xiangkai Zhuge, Fang Tang, Hongfei Zhu, Xiang Mao, Shaohui Wang, Zongfu Wu, Chengping Lu, Jianjun Dai, Hongjie Fan. AutA and AutR, Two Novel Global Transcriptional Regulators, Facilitate Avian Pathogenic Escherichia coli Infection. Scientific Reports, 2016, 6: 25085
16. Sandeep Kumar Misra, Francine Moussan Désirée Aké, Zongfu Wu, Eliane Milohanic, Thanh Nguyen Cao, Pascale Cossart, Josef Deutscher, Véronique Monnet, Cristel Archambaud, Céline Henry. Quantitative Proteome Analyses Identify PrfA-Responsive Proteins and Phosphoproteins in Listeria monocytogenes. Journal of Proteome Research, 2014, 13(12):6046-6057.
17. Jing Shao, Wei Zhang, Zongfu Wu, Chengping Lu. The Truncated Major Pilin
Subunit Sbp2 of the srtBCD Pilus Cluster Still Contributes to Streptococcus suis Pathogenesis in the Absence of Pilus Shaft. Current microbiology, 2014, 69(5):703-707.
18. Christophe Bécavin, Christiane Bouchier, Pierre Lechat, Cristel Archambaud, Sophie Creno, Edith Gouin, Zongfu Wu, Andreas Kühbacher, Sylvain Brisse, M. Graciela Pucciarelli, Francisco Garcí-del Portillo, Torsten Hain, Daniel A. Portnoy, Trinad Chakraborty, Marc Lecuit, Javier Pizarro-Cerdá, Ivan Moszer, Hélène Bierne, Pascale Cossart. Comparison of widely used Listeria monocytogenes strains EGD, 10403S, and EGD-e highlights genomic variations underlying differences in pathogenicity. mBio, 2014, 5(2):e00969-00914.
19. Yang Wang, Li Yi, Zongfu Wu, Jing Shao, Guangjin Liu, Hongjie Fan, Wei
Zhang, Chengping Lu. Comparative proteomic analysis of Streptococcus suis biofilms and planktonic cells that identified biofilm infection-related immunogenic proteins. PLoS One, 2012, 7(4):e33371.
20. Haodan Zhu, Dongyan Huang, Wei Zhang, Zongfu Wu, Yan Lu, Hongying Jia, Ming Wang, Chengping Lu. The novel virulence-related gene stp of
Streptococcus suis serotype 9 strain contributes to a significant reduction in mouse mortality. Microbial Pathogenesis, 2011, 51(6):442-53.
21. Yang Wang, Wei Zhang, Zongfu Wu, Chengping Lu.Reduced virulence is an important characteristic of biofilm infection of Streptococcus suis. FEMS microbiology letters, 2011, 316(1): 36-43.
22. Yang Wang, Wei Zhang, Zongfu Wu, Xianglei Zhu, Chengping Lu.Functional analysis of luxS in Streptococcus suis reveals a key role in
biofilm formation and virulence. Veterinary microbiology, 2011, 152 (1-2): 151-160.
BOOK
Swine Streptococcosis. Chengping Lu and Zongfu Wu (Editor). China Agriculture Press, 11.2015, ISBN 978-7-109-20686-1
PATENT
Method for constructing unmarked deletion mutant for Streptococcus suis strain GZ0565, China Patent, No. ZL 20121046716.5
GRANTS (PRINCIPAL APPLICANT)
1. 2018 Sino-French Outstanding Young Scientists Exchange Program, Ministry of Science and Technology of China.
2. Subproject leader of the National Key Research and Development Program of China, The etiology and epidemic characteristics of Streptococcus suis, 07.2017-12.2020, No. 2017YFD0500102
3. National Natural Science Foundation of China, The mechanism of small RNA
rss04 contributing to Streptococcus suis meningitis, 01.2016-12.2019, No. 31572544
4. Youth Foundation of National Natural Science Foundation of China, The pathogenesis mechanisms of a LysM containing protein in a Streptococcus suis serotype 9 meningitis isolate, 01.2012-12.2014, No.31101828
5. Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry of China, The pathogenesis mechanisms of DNA nuclease in a Streptococcus suis serotype 9 meningitis isolate, 05.2013-05.2015, No. 2013-693
6. The Natural Science Foundation of Jiangsu Province, The pathogenesis mechanisms of o-acetylserine lyase and other extracellular enzymes in a Streptococcus suis serotype 9 meningitis isolate, 07.2011-06.2014, No.BK2011644
7. Specialized Research Fund for the Doctoral Program of Higher Education of China,
The pathogenesis mechanisms of 5’-nucleotidase in a Streptococcus suis serotype 9 meningitis isolate, 01.2012-12.2014, No. 20110097120016
8. Fundamental Research Funds for the Central Universities, Effect of anti-phagocytosis of Mac-1 containing protein on Streptococcus suis virulence, 05.2013-05.2016, No. KYZ201314
AWARDS
1. 2013 5th FEMS Congress Grants for Young Scientists, Leipzig, Germany
2. 2009 Award for outstanding graduates of Nanjing Agricultural University, China
3. 2008 First-class Scholarship of Fort Dodge-China Animal Husbandry, Nanjing, China
4. 2007 First-class Scholarship of Boehringer-Ingelheim, Nanjing, China
ORAL COMMUNICATIONS
1. April 2018, “The new regulator of Streptococcus suis virulence: small RNAs”, Yangtze River Delta Doctorial Forum in Preventive Veterinary Medicine, Shanghai, China
2. September 2016, “Streptococcus suis Small RNA rss04 Contributes to Meningitis in Mouse Infection Model”, The 3rd International Workshop on Streptococcus suis, Braunschweig, Germany
3. March 2009, “Identifying immunogenic proteins and revealing putative virulence-associated factors of Streptococcus suis Serotype 9 ”. Institut Pasteur, Paris, France.
4. June 2008, “Comparative proteome analysis of secreted proteins of
Streptococcus suis serotype 9”. The 6th Annual Academic Doctoral Conference, Chongqing, China.
5. October 2008, “Transcriptome profiling of zebrafish due to Streptococcus suis infection”. Annual Academic Conference of Jiangsu Society for Microbiology, Nanjing, China.
6. October 2007, “Immunoproteomic assay of surface proteins of Streptococcus suis serotype 9”. Annual Academic Conference of Jiangsu Society for Microbiology, Nanjing, China.
