Basic Information
Name: Yan Ruofeng
Title: Professor
Telephone: +86-25-84395904
Email: yanruofeng@njau.edu.cn
Address: No.1 Weigang, Nanjing, 210095, P. R. China
Research Areas and Descriptors: Veterinary parasitology
Profile
Degrees
Bachelor, Veterinary medicine (Anhui Agricultural University, 1998)
Master, Preventive veterinary medicine (Anhui Agricultural University, 2001)
Ph.D., Veterinary parasitology (Nanjing Agricultural University, 2004)
Work Experiences
College of Veterinary Medicine, Nanjing Agricultural University (Teacher, 2004-)
Institute of Parasitology, McGill University, Canada (Visiting scholar, 2009-2010)
College of Veterinary Medicine, Xinjiang Agricultural University (Vice dean, 2014-2016)
Active Affiliations
Chinese Association of Veterinary Parasitology
Chinese Association of Food Sanitation
Chinese Association of Zoology
Jiangsu Association of Immunology
Research Interests
His research focused on the molecular biology and immunology of nematodes such as Haemonchus contortus, Trichinella spiralis, and Caenorhabditis elegans. He was also interested in the protozoa like Toxoplasma gondii and Eimeria. spp of chicken. RNAi, comparative proteomics, transcriptomics and other techniques were applied in studying of the interactions between parasites and the hosts. He has identified some novel genes that could be important in the survival for parasites. To evaluate the functions, these genes were cloned, expressed, and their protective potentialities were evaluated. His results showed that several different ways might be taken by parasites in the evasions.
Publications
***Haemonchus contortus
(1) Muhammad Ehsan, WenJuan Wang, Javaid Ali Gadahi, Muhammad Waqqas Hasan, MingMin Lu, YuJian Wang, XinChao Liu, Muhammad Haseeb, RuoFeng Yan, LiXin Xu, XiaoKai Song, XiangRui Li. The Serine/Threonine-Protein Phosphatase 1 From Haemonchus contortus Is Actively Involved in Suppressive Regulatory Roles on Immune Functions of Goat Peripheral Blood Mononuclear Cells. Frontiers in immunology, 2018, 9: 1627
(2) Baojie Li, Javaid Ali Gadahi, Wenxiang Gao, Zhenchao Zhang, Muhammad Ehsan, Lixin Xu, Xiaokai Song, Xiangrui Li, Ruofeng Yan*. Characterization of a novel aspartyl protease inhibitor from Haemonchus contortus. Parasites & Vectors, 2017, 10: 191
(3) Lu MingMin, Tian XiaoWei, Yang XinChao, Yuan Cheng, Ehsan Muhammad, Liu XinChao, Yan RuoFeng, Xu Lixin, Song XiaoKai, Li XiangRui*. The N- and C-terminal carbohydrate recognition domains of Haemonchus contortus galectin bind to distinct receptors of goat PBMC and contribute differently to its immunomodulatory functions in hostparasite interactions. PARASITES & VECTORS, 2017, 10: 409
(4) Wen YuLing, Wang YuJian, Wang WenJuan, Lu MingMin, Ehsan Muhammad, Tian XiaoWei, Yan RuoFeng, Song XiaoKai, Xu Lixin, Li XiangRui*. Recombinant Miro domain-containing protein of Haemonchus contortus (rMiro-1) activates goat peripheral blood mononuclear cells in vitro. VETERINARY PARASITOLOGY, 2017, 243: 100-104
(5) Wang Yujian, Wen Yuling, Wang Shuai, Ehsan Muhammad, Yan RuoFeng, Song XiaoKai, Xu Lixin, Li XiangRui*. Modulation of goat monocyte function by HCcyst-2, a secreted cystatin from Haemonchus contortus. ONCOTARGET, 2017,8(27): 44108-44120
(6) Ehsan Muhammad, Gao WenXiang, Gadahi Javaid Ali, Lu MingMin, Liu XinChao, Wang YuJian, Yan RuoFeng, Xu LiXin, Song XiaoKai, Li XiangRui*. Arginine kinase from Haemonchus contortus decreased the proliferation and increased the apoptosis of goat PBMCs in vitro. PARASITES & VECTORS, 2017, 10: 311
(7) Wang Yujian, Lu MingMin, Wang Shuai, Ehsan Muhammad, Yan RuoFeng, Song XiaoKai, Xu Lixin, Li XiangRui*. Characterization of a secreted macrophage migration inhibitory factor homologue of the parasitic nematode Haemonchus contortus acting at the parasite-host cell interface. ONCOTARGET, 2017, 8(25): 40052-40064
(8) Gadahi Javaid Ali, Ehsan Muhammad, Wang Shuai, Zhang Zhenchao, Yan Ruofeng, Song Xiaokai, Xu Lixin, Li Xiangrui*. Recombinant protein of Haemonchus contortus small GTPase ADP-ribosylation factor 1 (HcARF1) modulate the cell mediated immune response in vitro. ONCOTARGET, 2017, 8(68):112211-112221
(9) Fang Wang, Lixin Xu, Xiaokai Song, Xiangrui Li, Ruofeng Yan*. Identification of differentially expressed proteins between free-living and activated third-stage larvae of Haemonchus contortus. Veterinary parasitology, 2016, 215(1): 72-77
(10) Ruofeng Yan, Jingjing Wang, Lixin Xu, Xiaokai Song and Xiangrui Li. DNA vaccine encoding Haemonchus contortus. Actin induces partial protection in Goats. Acta Parasitologica, 2014, 59(4), 698-709
(11) Ruofeng Yan, Wei Sun, Xiaokai Song, Lixin Xu, Xiangrui Li. Vaccination of goats with DNA vaccine encoding Dim-1 induced partial protection against Haemonchus contortus : A preliminary experimental study. Research in Veterinary Science, 2013, 95: 189-199
(12) Zhikai Zhang, Lixin Xu, Xiaokai Song, Xiangrui Li, Ruofeng Yan*. Characterization of HcMSP, a novel gender specific gene from parasitic nematode Haemonchus contortus. Journal of Animal and Veterinary Advances, 2013, 12(5): 593-599
(13) Ruofeng Yan, Lixin Xu, Jingjing Wang, Xiaokai Song, Xiangrui Li*. Cloning and Characterization of Aldolase from Parasitic Nematode Haemonchus contortus. Journal of Animal and Veterinary Advances, 2013, 12(4): 478-486
(14) Ruofeng Yan, Ludmel Urdaneta-Marquez, Kathy Keller, Catherine E James, Mary W Davey, Roger K Prichard*. The role of several ABC transporter genes in ivermectin resistance in Caenorhabditis elegans. Veterinary Parasitology, 2012，190（3-4）：519-529
(15) GuangWei Zhao#, RuoFeng Yan#, Charles I. Muleke, YanMing Sun, LiXin Xu, XiangRui Li*. Vaccination of goats with DNA vaccines encoding H11 and IL-2 induces partial protection against Haemonchus contortus infection. The Veterinary Journal, 2012, 191: 94-100
(16) Javaid Ali Gadahi, Bu Yongqian, Muhammad Ehsan, ZhenChao Zhang, Shuai WANG, RuoFeng Yan, XiaoKai Song, LiXin Xu, XiangRui Li*. Haemonchus contortus excretory and secretory proteins (HcESPs) suppress functions of PBMCs in vitro. Oncotarget, 2016, 7(24): 35670-35679
(17) Gadahi JA, Wang S, Bo G, Ehsan M, Yan R, Song X, Xu L, Li X*. Proteomic Analysis of the Excretory and Secretory Proteins of Haemonchus contortus (HcESP) Binding to Goat PBMCs In Vivo Revealed Stage-Specific Binding Profiles. PLoS One, 2016, 11(7): e0159796
(18) Li Y, Yuan C, Wang L, Lu M, Wang Y, Wen Y, Yan R, Xu L, Song X, Li X*. Transmembrane protein 147 (TMEM147): another partner protein of Haemonchus contortus galectin on the goat peripheral blood mononuclear cells (PBMC). Parasites & Vectors, 2016, 9(1): 355
(19) Gadahi JA, Ehsan M, Wang S, Zhang Z, Wang Y, Yan R, Song X, Xu L, Li X*. Recombinant protein of Haemonchus contortus 14-3-3 isoform 2 (rHcftt-2) decreased the production of IL-4 and suppressed the proliferation of goat PBMCs in vitro. Exp Parasitol, 2016, 171: 57-66
(20) Javaid Ali Gadahi, Baojie Li, Muhammad Ehsan1, Shuai Wang, Zhenchao Zhang, Yujian Wang, Muhammad Waqqas Hasan, Ruofeng Yan, Xiaokai Song, Lixin Xu, Xiangrui Li*. Recombinant Haemonchus contortus 24 kDa excretory/ secretory protein (rHcES-24) modulate the immune functions of goat PBMCs in vitro. Oncotarget, 2016, 7(51): 83926-83937
(21) Yuan C, Zhang H, Wang W, Li Y, Yan R, Xu L, Song X, Li X*. Transmembrane protein 63A is a partner protein of Haemonchus contortus galectin in the regulation of goat peripheral blood mononuclear cells. Parasites & Vectors, 2015, 8: 211
(22) Wang W, Wang S, Zhang H, Yuan C, Yan R, Song X, Xu L, Li X*. Galectin Hco-gal-m from Haemonchus contortus modulates goat monocytes and T cell function in different patterns. Parasites & Vectors, 2014, 7: 342
(23) Wang W, Yuan C, Wang S, Song X, Xu L, Yan R, Hasson IA, Li X*. Transcriptional and proteomic analysis reveal recombinant galectins of Haemonchus contortus down-regulated functions of goat PBMC and modulation of several signaling cascades in vitro. J Proteomics, 2014, 98:123-37
(24) Wei Sun, Xiaokai Song, Ruofeng Yan, Lixin Xu, Xiangrui Li*. Cloning and characterization of a selenium-independent glutathione peroxidase (HC29) from adult Haemonchus contortus. J Vet Sci, 2012, 13(1), 49-58
(25) Kaikai Han, Lixin Xu, Ruofeng Yan, Xiaokai Song, Xiangrui Li*. Vaccination of goats with glyceraldehyde-3-phosphate dehydrogenase DNA vaccine induced partial protection against Haemonchus contortus. Veterinary Immunology and Immunopathology, 2012, 149(3-4): 177-185
(26) Kaikai Han, Lixin Xu, Ruofeng Yan, Xiaokai Song, Xiangrui Li*. Molecular cloning, expression and characterization of enolase from adult Haemonchus contortus. Research in Veterinary Science, 2012, 92 (2): 259-265
(27) Wei Sun, Xiaokai Song, Ruofeng Yan, Lixin Xu, Xiangrui Li*. Vaccination of goats with a glutathione peroxidase DNA vaccine induced partial protection against Haemonchus contortus infection. Veterinary Parasitology, 2011, 182: 239–247
(28) Han Kai kai, Xu Lixin, Yan Ruofeng, Song Xiaokai, Li Xiangrui*. Cloning, expression and characterization of NAD(+)-dependent glyceraldehyde-3-phosphate dehydrogenase of adult Haemonchus contortus. Journal of Helminthology，2011，85（4）：421-429
(29) Fengbin Yan, Lixin Xu, Liheng Liu, Ruofeng Yan, Xiaokai Song, Xiangrui Li*. Immunoproteomic analysis of whole proteins from male and female adult Haemonchus contortus. The Veterinary Journal, 2010, 185(2): 174-179
(30) YI Daosheng, XU Lixin, YAN Ruofeng, LI Xiangrui*. Haemonchus contortus: Cloning and characterization of serpin. Experimental Parasitology, 2010, 125(4): 363-370
(31) Yanming Sun, Ruofeng Yan, Charles I. Muleke, Guangwei Zhao, Lixin Xu, Xiangrui Li*. Recombinant galectins of Haemonchus contortus Parasite induces apoptosis in the peripheral blood lymphocytes of goat. International Journal of Peptide Research and Therapeutics, 2007, 13(3): 387-392
(32) Sun Yanming, Ruofeng Yan, Charles I. Mulek, Zhao Guangwei, Xu Lixin, Li Xiangrui*. Vaccination of goats with recombinant galectin antigen induces partial protection against Haemonchus contortus infection. Parasite Immunology, 2007, 29: 319-326
(33) Charles I. Muleke, Yan Ruofeng, Xu Lixin, Bo Xinwen, Li Xiangrui*. Cloning and sequence analysis of Hemonchus Contortus HC58 cDNA. DNA Sequence, 2007, 18(3): 176-183
(34) Charles I. Muleke, Yan Ruofeng, Sun Yanming, Zhao Guangwei, Xu lixin, Li Xiangrui*. Vaccination of goats against Haemonchus contortus with a recombinant cysteine Protease. Small Ruminant Research, 2007, 73: 95-102
(35) Jing Wang, Ruofeng Yan, Lixin Xu, Xiangrui Li*. The second glutamic acid in the C-terminal CRD affects the carbohydrate-binding properties of recombinant galectins of Haemonchus contortus. Veterinary Parasitology, 2007, 148 (3-4): 247-255
***Eimeria spp.
(36) Liu Tingqi, Huang Jingwei, Li Yanlin, Ehsan Muhammad, Wang Shuai, Zhou Zhouyang, Song Xiaokai, Yan Ruofeng, Xu Lixin, Li Xiangrui*. Molecular characterisation and the protective immunity evaluation of Eimeria maxima surface antigen gene. pARASITES & VECTORS, 2018,11:325
(37) Huang Jingwei, Liu Tingqi, Li Ke, Song Xiaokai, Yan Ruofeng, Xu Lixin, Li Xiangrui*. Proteomic analysis of protein interactions between Eimeria maxima sporozoites and chicken jejunal epithelial cells by shotgun LC-MS/MS. PARASITES & VECTORS, 2018, 11: 226
(38) Liu Tingqi, Huang Jingwei, Ehsan Muhammad, Wang Shuai, Fei Hong, Zhou Zhouyang, Song Xiaokai, Yan Ruofeng, Xu Lixin, Li Xiangrui*. Protective immunity against Eimeria maxima induced by vaccines of Em14-3-3 antigen. VETERINARY PARASITOLOGY, 2018, 253: 79-86
(39) Tian Lu, Li Wenyu, Huang Xinmei, Tian Di, Liu Jianhua, Yang Xinchao, Liu Lianrui, Yan RuoFeng, Xu Lixin, Li XiangRui, Song XiaoKai. Protective Efficacy of Coccidial Common Antigen Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) against Challenge with Three Eimeria Species. FRONTIERS IN MICROBIOLOGY, 2017, 8: 1245
(40) Liu Lianrui, Huang Xinmei, Liu Jianhua, Li Wenyu, Ji Yihong, Tian Di, Tian Lu, Yang Xinchao, Xu Lixin, Yan Ruofeng, Li Xiangrui, Song Xiaokai. Identification of common immunodominant antigens of Eimeria tenella, Eimeria acervulina and Eimeria maxima by immunoproteomic analysis. ONCOTARGET, 2017, 8(21): 34935-34945
(41) Song Xiaokai, Zhao Xiaofang, Xu Lixin, Yan Ruofeng, Li XiangRui. Immune protection duration and efficacy stability of DNA vaccine encoding Eimeria tenella TA4 and chicken IL-2 against coccidiosis. RESEARCH IN VETERINARY SCIENCE, 2017, 111: 31-35
(42) Yang XinChao, Li MengHui, Liu JianHua, Ji YiHong, Li XiangRui, Xu LiXin, Yan RuoFeng, Song XiaoKai. Identification of immune protective genes of Eimeria maxima through cDNA expression library screening. PARASITES & VECTORS, 2017, 10: 85
(43) Song X, Zhang Z, Liu C, Xu L, Yan R, Li X*. Evaluation of the persistence, integration, histopathology and environmental release of DNA vaccine encoding Eimeria tenella TA4 and chicken IL-2. Vet Parasitol. 2016, 229:22-30
(44) Zhang Z, Liu X, Yang X, Liu L, Wang S, Lu M, Ehsan M, Gadahi JA, Song X, Xu L, Yan R, Li X*. The Molecular Characterization and Immunity Identification of Microneme 3 of Eimeria acervulina. J Eukaryot Microbiol. 2016, doi: 10.1111/jeu.12318
(45) Zhang Z, Liu L, Huang J, Wang S, Lu M, Song X, Xu L, Yan R, Li X*. The molecular characterization and immune protection of microneme 2 of Eimeria acervulina. Vet Parasitol. 2016, 215:96-105
(46) Hoan TD, Zhang Z, Huang J, Yan R, Song X, Xu L, Li X*. Identification and immunogenicity of microneme protein 2 (EbMIC2) of Eimeria brunetti. Exp Parasitol. 2016, 162:7-17
(47) Xiaokai Song，Yunlu Gao，Lixin Xu，Ruofeng Yan，Xiangrui Li*. Partial protection against four species of chicken coccidia induced by multivalent subunit vaccine. Veterinary Parasitology，2015，212（3–4）：80-85
(48) Xiaokai Song#，Xinmei Huang#，Ruofeng Yan，Lixin Xu，Xiangrui Li*. Efficacy of chimeric DNA vaccines encoding Eimeria tenella 5401 and chicken IFN-γ or IL-2 against coccidiosis in chickens. Experimental Parasitology，2015，156：19-25
(49) Song, Xiaokai，Zhang, Ruirui，Xu, Lixin，Yan, Ruofeng，Li, Xiangrui*. Chimeric DNA vaccines encoding Eimeria acervulina macrophage migration inhibitory factor (E.MIF) induce partial protection against experimental Eimeria infection. Acta Parasitologica，2015，60（3）500-508
(50) Xiaokai Song，Lixin Xu，Ruofeng Yan，Xinmei Huang，Xiangrui Li*. Construction of Eimeria tenella multi-epitope DNA vaccines and their protective efficacies against experimental infection. Veterinary Immunology and Immunopathology，2015，166（3–4）：79-87
(51) Xiaokai Song，Zhe Ren，Ruofeng Yan，Lixin Xu，Xiangrui Li*. Induct ion of protective immunity against Eimeria tenella, Eimeria necatrix, Eimeriamaxima and Eimeria acervulina infections using multivalent epitope DNA vaccines. Vaccine，2015，33（24）：2764-2770
(52) Huang J, Zhang Z, Li M, Song X, Yan R, Xu L, Li X*. Immune protection of Microneme 7 (EmMIC7) against Eimeria maxima challenge in chickens. Avian Pathol. 2015, 16:1-35
(53) Huang J, Zhang Z, Li M, Song X, Yan R, Xu L, Li X*. Eimeria maxima microneme protein 2 delivered as DNA vaccine and recombinant protein induces immunity against experimental homogenous challenge. Parasitol Int. 2015, 64(5):408-416
(54) Zhang Z, Wang S, Huang J, Liu L, Lu M, Li M, Sui Y, Xu L, Yan R, Song X, Li X*. Proteomic analysis of Eimeria acervulina sporozoite proteins interaction with duodenal epithelial cells by shotgun LC-MS/MS. Mol Biochem Parasitol. 2015, 202(2):29-33
(55) Tran Duc Hoan，Doan Thi Thao，Gadahi, Javaid Ali，Song, Xiaokai，Xu, Lixin，Yan, Ruofeng，Li, Xiangrui*，Analysis of humoral immune response and cytokines in chickens vaccinated with Eimeria brunetti apical membrane antigen-1 (EbAMA1) DNA vaccine. Experimental Parasitology，2014，144：65-72
(56) Zhang Z, Huang J, Li M, Sui Y, Wang S, Liu L, Xu L, Yan R, Song X, Li X*. Identification and molecular characterization of microneme 5 of Eimeria acervulina. PLoS One. 2014, 9(12):e115411
(57) Hongyan Song#, Baofeng Qiu#, Ruofeng Yan, Lixin Xu, Xiaokai Song, Xiangrui Li*. The protective efficacy of chimeric SO7/IL-2 DNA vaccine against coccidiosis in chickens. Research in Veterinary Science, 2013, 94 (3): 562-567
(58) Zhu, Huili，Xu, Lixin，Yan, Ruofeng，Song, Xiaokai，Tang, Fang，Wang, Song，Li, Xiangrui*. Identification and characterization of a cDNA clone-encoding antigen of Eimeria acervulina. Parasitology，2012，139（13）：1711-1719
(59) Zhu, Huili，Yan, Ruofeng，Wang, Song，Song, Xiaokai，Xu, Lixin，Li,Xiangrui*. Identification and molecular characterization of a novel antigen of Eimeria acervulina，Molecular and Biochemical Parasitology，2012，186（1）：21-28
(60) Shah, Muhammad Ali A.，Song, Xiaokai，Xu, Lixin，Yan, Ruofeng，Li, Xiangrui*. Construction of DNA vaccines encoding Eimeria acervulina cSZ-2 with chicken IL-2 and IFN-gamma and their efficacy against poultry coccidiosis. Research in Veterinary Science，2011，90（1）：72-77
(61) Song, Hongyan，Yan, Ruofeng，Xu, Lixin，Song, Xiaokai，Shah, Muhammad Ali A.，Zhu, Huili，Li, Xiangrui*. Efficacy of DNA vaccines carrying Eimeria acervulina lactate dehydrogenase antigen gene against coccidiosis. Experimental Parasitology，2010，126（2）：224-231
(62) Shah, Mohammad Ali A.，Yan, Roufeng，Xu, Yan Lixin，Song, Xiaokai，Li, Xiangrui*. A recombinant DNA vaccine encoding Eimeria acervulina cSZ-2 induces immunity against experimental E. tenella infection. Veterinary Parasitology，2010，169（1-2）：185-189
(63) Song, Hongyan，Song, Xiaokai，Xu, Lixin，Yan, Ruofeng，Shah, Muhammad Ali A.，Li, Xiangrui*. Changes of cytokines and IgG antibody in chickens vaccinated with DNA vaccines encoding Eimeria acervulina lactate dehydrogenase. Veterinary Parasitology，2010，173（3-4）：219-227
(64) Shah, Muhammad Ali A.，Song, Xiaokai，Xu, Lixin，Yan, Roufeng，Song, Hongyan，Ruirui, Zhang，Chengyu, Lei，Li, Xiangrui*. The DNA-induced protective immunity with chicken interferon gamma against poultry coccidiosis. Parasitology Research，2010，107（3）：747-750
(65) Shah, Mohammed Ali A.，Xu, Lixin，Yan, Ruofeng，Song, Xiaokai，Li,Xiangrui*. Cross immunity of DNA vaccine pVAX1-cSZ2-IL-2 to Eimeria tenella, E. necatrix and E. maxima. Experimental Parasitology，2010，124（3）：330-333
(66) Liu, Liheng，Xu, Lixin，Yan, Fengbin，Yan, Ruofeng，Song, Xiaokai，Li , Xiangrui*. Immunoproteomic analysis of the second-generation merozoite proteins of Eimeria tenella，Veterinary Parasitology，2009，164（2-4）：173-182
(67) Song, Xiaokai，Xu, Lixin，Yan, Ruofeng，Huang, Xinmei，Shah，Mohammed Ali A.，Li, Xiangrui*. The optimal immunization procedure of DNA vaccine pcDNA-TA4-IL-2 of Eimeria tenella and its cross-immunity to Eimeria necatrix and Eimeria acervulina. Veterinary Parasitology，2009，159（1）：30-36
(68) Xu, Qianming#，Song, Xiaokai#，Xu, Lixin，Yan, Ruofeng，Shah, Mohammed Ali A.，Li, Xiangrui*. Vaccination of chickens with a chimeric DNA vaccine encoding Eimeria tenella TA4 and chicken IL-2 induces protective immunity against coccidiosis. Veterinary Parasitology，2008，156（3-4）：319-323
***Toxoplasma gondii
(69) Liu Xinchao, Li Xiaoyu, Wang Qiangqiang, Sun Xiaoni, Lu Mingmin, Ehsan Muhammad, Xu Lixin, Yan RuoFeng, Song XiaoKai, Li XiangRui*. Toxoplasma gondii Histone 4 Affects Some Functions of Murine Ana-1 Macrophages In Vitro. The Journal of eukaryotic microbiology, 2018, DOI:10.1111/jeu.12630
(70) Wang Shuai, Zhang Zhenchao, Wang Yujian, Gadahi Javaid Ali, Xie Qing, Xu Lixin, Yan Ruofeng, Song Xiaokai, Li Xiangrui. Toxoplasma gondii excretory/secretory antigens (TgESAs) suppress pro-inflammatory cytokine secretion by inhibiting TLR-induced NF-kappa B activation in LPS-stimulated murine macrophages. ONCOTARGET, 2017, 8(51):88351-88359
(71) Liu Xinchao, Ma Qunshan, Sun Xiaoni, Lu Mingmin, Ehsan Muhammad, Hasan Muhammad Waqqas, Xu Lixin, Yan RuoFeng, Song XiaoKai, Li XiangRui. Effects of Recombinant Toxoplasma gondii Citrate Synthase I on the Cellular Functions of Murine Macrophages In vitro. FRONTIERS IN MICROBIOLOGY, 2017, 8: 1376
(72) Liu Xin-Chao, He Yu, Han Deng-Ge, Zhang Zhen-Chao, Li Ke, Wang Shuai, Xu Li-Xin, Yan Ruo-Feng, Li Xiang-Rui. Detection of Toxoplasma gondii in chicken and soil of chicken farms in Nanjing region, China. INFECTIOUS DISEASES OF POVERTY, 2017, 6: 62
(73) Wang S, Hassan IA, Liu X, Xu L, Yan R, Song X, Li X*. Immunological changes induced by Toxoplasma gondii Glutathione-S-Transferase (TgGST) delivered as a DNA vaccine. Res Vet Sci. 2015, 99:157-164
(74) Wang S, Wang Y, Sun X, Zhang Z, Liu T, Gadahi JA, Hassan IA, Xu L, Yan R, Song X, Li X*. Protective immunity against acute toxoplasmosis in BALB/c mice induced by a DNA vaccine encoding Toxoplasma gondii elongation factor 1-alpha. BMC Infect Dis. 2015,15:448
(75) Wang S, Wang Y, Sun X, Zhang Z, Liu T, Gadahi JA, Xu L, Yan R, Song X, Li X*. Protective immunity against acute toxoplasmosis in BALB/c mice induced by a DNA vaccine encoding Toxoplasma gondii 10 kDa excretory-secretory antigen (TgESA10). Vet Parasitol. 2015, 214(1-2):40-48
(76) Wang Shuai, Zhang Meng, Liu Xin-chao, Lin Tao, Yang Han-chun, Yuan Shi-shan, Zhao Guang-wei, Ia Hassan, Yan Ruo-feng, Song Xiao-kai, Li Xiang-rui*. Investigation on the co-infections of Toxoplasma gondii with PRRSV, CSFV or PCV-2 in swine in part of China. JOURNAL OF INTEGRATIVE AGRICULTURE, 2015, 14(9): 1838-1844
(77) Wang, Shuai, Zhao, Guang-Wei, Wang, Wang, Zhang, Zhen-Chao, Shen, Bo, Hassan, I. A., Xie, Qing, Yan, Ruo-Feng, Song, Xiao-Kai, Xu, Li-Xin, Li, Xiang-Rui*. Pathogenicity of Five Strains of Toxoplasma gondii from Different Animals to Chickens. KOREAN JOURNAL OF PARASITOLOGY, 2015,53(2):155-162
(78) Zhao Guang-wei, Wang Shuai, Wang Wang, Zhang Zhen-chao, Xie Qing, Zhang Meng, Hassan, I. A., Yan Ruo-feng, Song Xiao-kai, Xu Li-xin, Li Xiang-rui*. Type I strain of Toxoplasma gondii from chicken induced different immune responses with that from human, cat and swine in chicken. JOURNAL OF INTEGRATIVE AGRICULTURE. 2015,14(5): 956-965
(79) Ibrahim A Hassan, Shuai Wang, LiXin Xu, RuoFeng Yan, XiaoKai Song and Xiangrui Li*. DNA vaccination with a gene encoding Toxoplasma gondii Deoxyribose Phosphate Aldolase (TgDPA) induces partial protective immunity against lethal challenge in mice. Parasites & Vectors 2014, 7:431
(80) Ibrahim A. Hassan, Shuai Wang, LiXin Xu, RuoFeng Yan, XiaoKai Song, XiangRui Li*. Immunoglobulin and cytokine changes induced following immunization with a DNA vaccine encoding Toxoplasma gondii selenium-dependent glutathione reductase protein. Experimental Parasitology, 2014, 146: 1–10
(81) I. A. HASSAN, S. WANG, L. XU, R. YAN, X. SONG & L. XIANGRUI*. Immunological response and protection of mice immunized with plasmid encoding Toxoplasma gondii glycolytic enzyme malate dehydrogenase. Parasite Immunology, 2014, 36, 674–683
(82) Zhang M, Yang Z, Wang S, Tao L, Xu L, Yan R, Song X, Li X*. Detection of Toxoplasma gondii in shellfish and fish in parts of China. Vet Parasitol, 2014, 200(1-2):85-89
(83) Shuai Wang, GuangWei Zhao, Wang Wang, Qing Xie, Meng Zhang, Cheng Yuan, I. A. Hassan, XinChao Liu, LiXin Xu, RuoFeng Yan, XiaoKai Song, XiangRui Li*. Pathogenicity of two Toxoplasma gondii strains to chickens of different ages infected via intraperitoneal injection. Avian Pathology, 2014, 43(1): 1-5
(84) Guang-wei ZHAO, Bo SHEN, Qing XIE, Li-xin XU, Ruo-feng YAN, Xiao-kai SONG, Hassan Ibrahim Adam, Xiang-rui LI*. Isolation and Molecular Characterization of Toxoplasma gondii from Chickens in China. Journal of Integrative Agriculture, 2012, 11(8): 1347-1353
(85) GuangWei Zhao, Bo Shen, Qing Xie, Li Xin Xu, Ruo Feng Yan, Xiao Kai Song, I.A. Hassan, Xiang Rui Li. Detection of Toxoplasma gondii in free-range chickens in China based on circulating antigens and antibodies. Veterinary Parasitology, 2012, 185（2–4）：72-77
***Trichinella spiralis
(86) Hai Xing, Lixin Xu, Xiaokai Song, Xiangrui Li, Ruofeng Yan*. Seroprevalence of Toxoplasma gondii and Trichinella spiralis in Horses in Xinjiang, Northwestern China. Journal of Equine Veterinary Science, 2018, 60:11-15
(87) Xuliang Zhang, Lixin Xu, Xiaokai Song, Xiangrui Li, Ruofeng Yan*. Molecular cloning of enolase from Trichinella spiralis and the protective immunity in mice. Acta Parasitologica, 2018, 63(2): 252-260
(88) F. Tang, L. Xu, R. Yan, X. Song and X. Li*. A DNA vaccine co-expressing Trichinella spiralis MIF and MCD-1 with murine ubiquitin induces partial protective immunity in mice. Journal of Helminthology, 2013, 87(1): 24-33
(89) F. Tang, L. Xu, R. Yan, X. Song and X. Li*. Evaluation of the immune response induced by DNA vaccines expressing MIF and MCD-1 genes of Trichinella spiralis in BALB/c mice. Journal of Helminthology, 2012, 86(4):430-439
